分享产品介绍 FAQs 评论(0)
宿主来源
Mouse分子别名
Receptor-type tyrosine-protein phosphatase C; Leukocyte common antigen (L-CA); T200; PTPRC细胞定位
Cell membraneAccession
P08575克隆号
UCHL1抗体类型
Mouse mAb抗体同种型
IgG2a,k反应种属 ?
Hu纯化方式
Protein A浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
FCM
ICC ?
WB
IF ?
稀释度
应用 稀释度 WB 1:1000 IHC-P 1:1000 ICC 1:500 IF 1:200 FCM 1:2000
CD45RO is an important cell surface marker in immunology that belongs to the CD45 family (also known as Leukocyte Common Antigen, LCA). CD45 is a highly polymorphic transmembrane protein primarily expressed on the surface of white blood cells, including T cells, B cells, natural killer (NK) cells, monocytes, and granulocytes, and plays a crucial role in lymphocyte development, activation, and signaling. Specifically, CD45RO is an isoform of CD45 that is highly expressed on memory T cells but expressed at lower levels or not expressed on naive T cells. Memory T cells are T cells that have been activated and survived during previous immune responses, enabling them to rapidly and strongly activate upon re-encountering the same antigen, thus accelerating and enhancing immune responses. Therefore, CD45RO is often used to distinguish and identify memory T cells. It serves as an important marker in immune phenotyping analysis, disease diagnosis, immune response monitoring, and immunotherapy research.
免疫印迹
WB result of CD45RO Recombinant Mouse mAb
Primary antibody: CD45RO Mouse mAb at 1/1000 dilution
Lane 1: Jurkat whole cell lysate 20 µg
Lane 2: HuT 78 whole cell lysate 20 µg
Negative control: Jurkat whole cell lysate
Secondary antibody: Goat Anti-mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 180 kDa
Observed MW: 180 kDa
流式分析
Flow cytometric analysis of Jurkat (Human T cell leukemia T lymphocyte, left) / HuT 78 (Human Sezary syndrome cutaneous T lymphocyte, Right) labelling Human CD45RO antibody at 1/2000 dilution (0.1 μg) / (Red) compared with a Mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Mouse IgG Alexa Fluor® 488 was used as the secondary antibody.
Negative control: JurkatFlow cytometric analysis of human PBMC (human peripheral blood mononuclear cell) labelling CD45RO antibody at 1/2000 (0.1 μg) dilution (Right) compared with a Mouse monoclonal IgG isotype control (Left). Goat Anti - Mouse IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD45RA - PE/Cy5.5 separately. Gated on viable lymphocytes.
免疫组化
IHC shows positive staining in paraffin-embedded human spleen. Anti-CD45RO antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-CD45RO antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human cerebral cortex. Anti-CD45RO antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human thyroid cancer. Anti-CD45RO antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded non-Hodgkin’s T-cell lymphoma. Anti-CD45RO antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human anaplastic large cell lymphoma. Anti-CD45RO antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in HuT 78 cells (top panel) and negative staining in Jurkat cells (below panel). Anti-CD45RO antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
免疫荧光
IF shows positive staining in paraffin-embedded human tonsil. Anti-CD45RO antibody was used at 1/200 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded human anaplastic large cell lymphoma. Anti-CD45RO antibody was used at 1/200 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
评论(0)