热搜词：
注册
登录
CN
  • CN
  • EN

产品中心 抗体 其他一抗

所有图片（1/5）
申请试用

Apolipoprotein E/APOE Recombinant Rabbit mAb (S-970-75)

Apo-E

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B0703
规格
数量
收藏 分享

产品介绍 FAQs 引用文献(1) 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 分子别名

    Apo-E

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Secreted

  • Accession

    P02649

  • 克隆号

    S-970-75

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 反应种属 ?

    Hu

  • 预测反应种属
    (反应种属缩写表)

    Pr, Or

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    IHC-P ? ,
    WB

  • 稀释度

    应用 稀释度
    WB 1:1000
    IHC-P 1:500
背景介绍

  • Apolipoprotein E (ApoE) is a polymorphic protein that plays a vital role in the metabolism and transport of lipids in the body. Specifically, ApoE is a major component of plasma lipoproteins, including chylomicrons (CM), very low-density lipoproteins (VLDL), intermediate-density lipoproteins (IDL), and some high-density lipoproteins (HDL). ApoE is primarily synthesized in the liver, but it is also produced in other tissues such as the brain, kidneys, bones, adrenal glands, and macrophages. It participates in the transport, storage, and excretion of lipids, including cholesterol and triglycerides. It also serves as a ligand for low-density lipoprotein (LDL) receptors and chylomicron remnant receptors on hepatocytes, facilitating the clearance of these lipoproteins from the circulation. This process plays a crucial role in maintaining lipid homeostasis. Furthermore, ApoE has been implicated in various other biological processes, including neuroprotection, immune regulation, and tissue repair. Recent studies have suggested that ApoE may play a role in the development of neurological disorders such as Alzheimer's disease.

  • 免疫印迹

    • WB result of Apolipoprotein E/APOE Rabbit mAb
      Primary antibody: Apolipoprotein E/APOE Rabbit mAb at 1/1000 dilution
      Lane 1: HepG2 whole cell lysate 20 µg
      Lane 2: human plasma lysate 5 µg
      Lane 3: human serum lysate 5 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 36 kDa
      Observed MW: 35 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human tonsil. Anti- Apolipoprotein E/APOE antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human spleen. Anti- Apolipoprotein E/APOE antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human liver. Anti- Apolipoprotein E/APOE antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human testis. Anti- Apolipoprotein E/APOE antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

FAQs

斯达特公司的抗体，可以回收利用几次？

我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定

引用文献(1)

评论(0)