AICDA Recombinant Rabbit mAb (S-1030-15)

Single-stranded DNA cytosine deaminase,Activation-induced cytidine deaminase (AID),Cytidine aminohydrolase,AID

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0702

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
分子别名

Single-stranded DNA cytosine deaminase, Activation-induced cytidine deaminase (AID), Cytidine aminohydrolase, AID
免疫原

Synthetic Peptide
细胞定位

Cytoplasm, Nucleus
Accession

Q9GZX7
克隆号

S-1030-15
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu
预测反应种属
(反应种属缩写表)

Bv, Dg
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

WB

IP
稀释度

应用稀释度

WB 1:1000
IP 1:50
IHC-P 1:100

背景介绍

The AICDA protein, also known as Activation-Induced Cytidine Deaminase (AID), plays a crucial role in the immune system. As an enzyme, it mediates affinity maturation and promotes DNA demethylation in germinal center B cells. Specifically, AICDA is essential for generating antibody diversity and forming immune memory by inducing mutations and recombination in antibody genes. It facilitates somatic hypermutation and class switch recombination in immunoglobulin genes, thus enhancing the diversity and specificity of antibodies. However, abnormal expression of AICDA has also been associated with certain diseases. For instance, overexpression of AICDA has been shown to render BCL2-driven mouse lymphomas more aggressive. Additionally, excessive expression of AICDA is considered an important source of epigenetic heterogeneity in diffuse large B-cell lymphoma (DLBCL), which is primarily associated with relative loss of cytosine methylation, increasing tumor heterogeneity and overall adaptive capacity.

免疫印迹
- WB result of AICDA Rabbit mAb
  Primary antibody: AICDA Rabbit mAb at 1/1000 dilution
  Lane 1: K562 whole cell lysate 20 µg
  Lane 2: Daudi whole cell lysate 20 µg
  Lane 3: Ramos whole cell lysate 20 µg
  Lane 4: Raji whole cell lysate 20 µg
  Negative control: K562 whole cell lysate
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 24 kDa
  Observed MW: 24 kDa
免疫沉淀
- AICDA Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating AICDA in 0.4 mg Daudi whole cell lysate.
  Western blot was performed on the immunoprecipitate using AICDA Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/1000 dilution.
  Lane 1: Daudi whole cell lysate 20 µg (Input)
  Lane 2: AICDA Rabbit mAb IP in Daudi whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in Daudi whole cell lysate
  Predicted MW: 24 kDa
  Observed MW: 24 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human tonsil. Anti-AICDA antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.