所有图片（1/3）

TRIM24 Recombinant Rabbit mAb (S-1029-77)

Transcription intermediary factor 1-alpha,TIF1-alpha,E3 ubiquitin-protein ligase TRIM24,RING finger protein 82,RING-type E3 ubiquitin transferase TIF1-alpha,Tripartite motif-containing protein 24,RNF82,TIF1,TIF1A

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0701

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
分子别名

Transcription intermediary factor 1-alpha, TIF1-alpha, E3 ubiquitin-protein ligase TRIM24, RING finger protein 82, RING-type E3 ubiquitin transferase TIF1-alpha, Tripartite motif-containing protein 24, RNF82, TIF1, TIF1A
免疫原

Synthetic Peptide
细胞定位

Cytoplasm, Nucleus
Accession

O15164
克隆号

S-1029-77
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICFCM

ICC ?

WB
稀释度

应用稀释度

WB 1:1000
ICC 1:100
ICFCM 1:500

应用	稀释度
WB	1:1000
ICC	1:100
ICFCM	1:500

背景介绍

TRIM24 protein, also known as transcriptional intermediary factor 1 alpha (TIF1α), is a multifunctional co-activator protein that interacts dynamically with nuclear receptors and co-activators to influence target gene transcription. This protein belongs to the TRIM family of proteins, which are characterized by a conserved domain structure that includes RING, B-box, and coiled-coil regions. TRIM24 protein interacts with chromatin by recognizing specific histone H3 modifications, showing a high affinity for unmodified lysine 4 (H3K4me0) and acetylated lysine 23 (H3K23ac) of histone H3. This interaction is mediated by its bromodomain, which functions as a "reader" of epigenetic histone marks and regulates chromatin structure and gene expression by connecting relevant proteins to recognized acetylated histone targets. TRIM24 protein is an E3 ubiquitin protein ligase that promotes the proteasomal degradation of p53/TP53 and mediates cell proliferation and apoptosis along with TRIM33. It also regulates the transcriptional activation of retinoic acid (RA) receptors, including RARA, and has been shown to modulate RA-dependent hepatocyte proliferation. In addition, TRIM24 is the first transcription regulatory factor found to have a receptor target located in the nucleus. It can affect the expression and function of proteins related to chromatin remodeling by regulating them. TRIM24 can also utilize retinoic acid (RA) transcription to control tumorigenesis.

免疫印迹
- WB result of TRIM24 Rabbit mAb
  Primary antibody: TRIM24 Rabbit mAb at 1/1000 dilution
  Lane 1: HCT 116 whole cell lysate 20 µg
  Lane 2: HeLa whole cell lysate 20 µg
  Lane 3: COLO 205 whole cell lysate 20 µg
  Lane 4: IMR-32 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 116 kDa
  Observed MW: 140 kDa This blot was developed with high sensitivity substrate.
流式分析
- Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) labelling TRIM24 antibody at 1/500 dilution (0.1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫细胞化学
- ICC shows positive staining in HeLa cells. Anti-TRIM24 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).