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IL-8 Recombinant Rabbit mAb (S-162-38)

Interleukin-8,C-X-C motif chemokine 8,Chemokine (C-X-C motif) ligand 8,Emoctakin,Granulocyte chemotactic protein 1 (GCP-1),Monocyte-derived neutrophil chemotactic factor (MDNCF),Monocyte-derived neutrophil-activating peptide (MONAP),Neutrophil-activating protein 1 (NAP-1),Protein 3-10C,T-cell chemotactic factor,CXCL8

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0698

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

IL-8
分子别名

Interleukin-8, C-X-C motif chemokine 8, Chemokine (C-X-C motif) ligand 8, Emoctakin, Granulocyte chemotactic protein 1 (GCP-1), Monocyte-derived neutrophil chemotactic factor (MDNCF), Monocyte-derived neutrophil-activating peptide (MONAP), Neutrophil-activating protein 1 (NAP-1), Protein 3-10C, T-cell chemotactic factor, CXCL8
免疫原

Recombinant Protein
细胞定位

Secreted
Accession

P10145
克隆号

S-162-38
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICC ?

WB

IP
稀释度

应用稀释度

WB 1:1000
IP 1:50
ICC 1:500

应用	稀释度
WB	1:1000
IP	1:50
ICC	1:500

背景介绍

Interleukin 8 (IL-8 or chemokine (C-X-C motif) ligand 8, CXCL8) is a chemokine produced by macrophages and other cell types such as epithelial cells, airway smooth muscle cells and endothelial cells. Endothelial cells store IL-8 in their storage vesicles, the Weibel-Palade bodies. IL-8 has two primary functions. It induces chemotaxis in target cells, primarily neutrophils but also other granulocytes, causing them to migrate toward the site of infection. IL-8 also stimulates phagocytosis once they have arrived. IL-8 is also known to be a potent promoter of angiogenesis. In target cells, IL-8 induces a series of physiological responses required for migration and phagocytosis, such as increases in intracellular Ca2+, exocytosis (e.g. histamine release), and the respiratory burst. Interleukin-8 is a key mediator associated with inflammation where it plays a key role in neutrophil recruitment and neutrophil degranulation. As an example, it has been cited as a proinflammatory mediator in gingivitis and psoriasis.

免疫印迹
- WB result of IL-8 Recombinnat Rabbit mAb
  Primary antibody: IL-8 Rabbit mAb at 1/1000 dilution
  Lane 1: untreated PC-3 whole cell lysate 20 µg
  Lane 2: PC-3 treated with 2 µg/ml LPS and 500 ng/ml Brefeldin A for 5 hours whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 11 kDa
  Observed MW: 12 kDa
免疫沉淀
- IL-8 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating IL-8 in 0.4 mg PC-3 treated with 2 µg/ml LPS and 500 ng/ml Brefeldin A for 5 hours whole cell lysate.
  Western blot was performed on the immunoprecipitate using IL-8 Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/1000 dilution.
  Lane 1: PC-3 treated with 2 µg/ml LPS and 500 ng/ml Brefeldin A for 5 hours whole cell lysate 20 µg (Input)
  Lane 2: IL-8 Rabbit mAb IP in PC-3 treated with 2 µg/ml LPS and 500 ng/ml Brefeldin A for 5 hours whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in PC-3 treated with 2 µg/ml LPS and 500 ng/ml Brefeldin A for 5 hours whole cell lysate
  Predicted MW: 11 kDa
  Observed MW: 12 kDa
  This blot was developed with high sensitivity substrate
免疫细胞化学
- ICC analysis of PC-3 cells treated with LPS (2μg/ml, 5hr) + Brefeldin A (500ng/ml, 5hr) (top panel) and untreated PC-3 cells (below panel). Anti- IL-8 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).