HLA-E Recombinant Rabbit mAb (S-1081-62)

HLA class I histocompatibility antigen alpha chain E,MHC class I antigen E,HLA-6.2,HLAE

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0697

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
分子别名

HLA class I histocompatibility antigen alpha chain E, MHC class I antigen E, HLA-6.2, HLAE
免疫原

Synthetic Peptide
细胞定位

Cell membrane
Accession

P13747
克隆号

S-1081-62
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms
预测反应种属
(反应种属缩写表)

Cz
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms
IHC-P 1:500-1:2000 Hu, Ms

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms
IHC-P	1:500-1:2000	Hu, Ms

背景介绍

HLA-E is a non-classical MHC class I molecule that exhibits limited polymorphism and lower cell surface expression compared to its classical counterparts. HLA-E plays a unique role in immune responses. It can present antigen epitopes derived from pathogens, tumors, and self-antigens to CD8+ T cells for recognition by their T cell receptors (TCRs), mediating CD8+ T cell responses. HLA-E can interact with inhibitory receptors CD94/NKG2A/C on NK cells and some T cells, regulating their killing activities. Recent research has found that HLA-E-restricted T cells are able to recognize and destroy infected cells, playing a crucial role in the immune response against COVID-19. HLA-E was found to be resistant to inhibition by the virus, even though other classical HLA proteins were suppressed. When HLA-E-restricted T cells recognize the presented antigen fragments, they become activated and release cytotoxic factors to destroy the infected cells.

免疫印迹
- WB result of HLA-E Rabbit mAb
  Primary antibody: HLA-E Rabbit mAb at 1/1000 dilution
  Lane 1: THP-1 whole cell lysate 20 µg
  Lane 2: Jurkat whole cell lysate 20 µg
  Lane 3: HL-60 whole cell lysate 20 µg
  Lane 4: Daudi whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 40 kDa
  Observed MW: 40 kDa
- WB result of HLA-E Rabbit mAb
  Primary antibody: HLA-E Rabbit mAb at 1/1000 dilution
  Lane 1: mouse spleen lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 40 kDa
  Observed MW: 50 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human liver. Anti- HLA-E antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti- HLA-E antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human kidney. Anti- HLA-E antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human spleen. Anti- HLA-E antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human tonsil. Anti- HLA-E antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human ovarian carcinoma Anti- HLA-E antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human gastric cancer. Anti- HLA-E antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human transitional cell carcinoma Anti- HLA-E antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse colon. Anti- HLA-E antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse liver. Anti- HLA-E antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.