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Mouse细胞定位
Cytoplasm, CytoskeletonAccession
P10636克隆号
AT8抗体类型
Mouse mAb抗体同种型
IgG1,k应用
IHC-P, WB反应种属 ?
Ms纯化方式
Protein G浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 |
|---|---|
| WB | 1:1000 |
| IHC-P | 1:500 |
Tau protein is a microtubule-associated protein that plays a crucial role in the stabilization and function of microtubules in neurons. Abnormal phosphorylation of tau protein, including at the S202 and T205 sites, is associated with the development of tau-related neurodegenerative diseases such as Alzheimer's disease (AD). Phosphorylation of tau protein at specific sites can alter its function and interaction with microtubules, leading to their destabilization and dysfunction. The accumulation of abnormally phosphorylated tau protein, often referred to as neurofibrillary tangles, is a hallmark pathological feature of AD and other tauopathies. In AD, tau phosphorylation at the S202 and T205 sites has been found to increase significantly in affected brain regions. This abnormal phosphorylation is thought to contribute to the disruption of microtubule networks, impairment of axonal transport, and ultimately, neuronal dysfunction and death.
免疫印迹
WB result of Tau (phospho S202+T205) Mouse mAb
Primary antibody: Tau (phospho S202+T205) Mouse mAb at 1/1000 dilution
Lane 1: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 50~80 kDa
Observed MW: 50 kDa
免疫组化
IHC shows positive staining in paraffin-embedded mouse cerebral cortex (Left) and negative staining in phosphatase treated (37°C, 2hr) paraffin-embedded mouse cerebral cortex (Right). Anti-Tau (phospho S202+T205) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
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