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Bad Recombinant Rabbit mAb (S-976-26)

Bcl2-associated agonist of cell death,Bcl-2-binding component 6,Bcl-2-like protein 8 (Bcl2-L-8),Bcl-xL/Bcl-2-associated death promoter,Bcl2 antagonist of cell death,BBC6,BCL2L8

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0686

规格

25μl100μl1ml

数量

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
分子别名

Bcl2-associated agonist of cell death, Bcl-2-binding component 6, Bcl-2-like protein 8 (Bcl2-L-8), Bcl-xL/Bcl-2-associated death promoter, Bcl2 antagonist of cell death, BBC6, BCL2L8
免疫原

Synthetic Peptide
细胞定位

Cytoplasm
Accession

Q92934
克隆号

S-976-26
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ? ,
WB ,
IP
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms, Rt
IP 1:50 Hu
IHC-P 1:250 Hu, Ms, Rt

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms, Rt
IP	1:50	Hu
IHC-P	1:250	Hu, Ms, Rt

背景介绍

BAD protein, or Bcl-2-associated death promoter, is a member of the Bcl-2 protein family that is involved in the regulation of apoptosis (cell death). It functions as a pro-apoptotic protein, promoting cell death when activated. BAD protein interacts with other Bcl-2 family members, such as Bcl-2 and Bcl-xL, to regulate the release of cytochrome c from the mitochondria, which is a crucial step in the apoptotic cascade. The activity of BAD protein is regulated by phosphorylation. When BAD is phosphorylated, it dissociates from Bcl-2 and Bcl-xL, losing its pro-apoptotic function. Conversely, when BAD is dephosphorylated, it binds to Bcl-2 and Bcl-xL, inhibiting their anti-apoptotic activity and promoting apoptosis. The phosphorylation of BAD protein is mediated by several kinases, including AKT (also known as protein kinase B). AKT phosphorylates BAD at specific sites, preventing its interaction with Bcl-2 and Bcl-xL and thus inhibiting apoptosis. This mechanism is important in promoting cell survival and proliferation in response to growth factors and other stimuli.

免疫印迹
- WB result of Bad Rabbit mAb
  Primary antibody: Bad Rabbit mAb at 1/1000 dilution
  Lane 1: HeLa whole cell lysate 20 µg
  Lane 2: MCF7 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 18 kDa
  Observed MW: 20 kDa
- WB result of Bad Rabbit mAb
  Primary antibody: Bad Rabbit mAb at 1/1000 dilution
  Lane 1: C2C12 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 18 kDa
  Observed MW: 20 kDa
  (This blot was developed with high sensitivity substrate)
- WB result of Bad Rabbit mAb
  Primary antibody: Bad Rabbit mAb at 1/1000 dilution
  Lane 1: C6 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 18 kDa
  Observed MW: 20 kDa
免疫沉淀
- Bad Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Bad in 0.4 mg HeLa whole cell lysate.
  Western blot was performed on the immunoprecipitate using Bad Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/1000 dilution.
  Lane 1: HeLa whole cell lysate 20 µg (Input)
  Lane 2: Bad Rabbit mAb IP in HeLa whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in HeLa whole cell lysate
  Predicted MW: 18 kDa
  Observed MW: 20 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human colon. Anti-Bad antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human kidney. Anti-Bad antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative control: IHC shows negative staining in paraffin-embedded human skeletal muscle. Anti-Bad antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human colon cancer. Anti-Bad antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse kidney. Anti-Bad antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat kidney. Anti-Bad antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.