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CD52 Recombinant Mouse mAb (S-R431)

CAMPATH-1 antigen,CDw52,Cambridge pathology 1 antigen,Epididymal secretory protein E5,Human epididymis-specific protein 5 (He5),HE5

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0685

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Mouse
分子别名

CAMPATH-1 antigen, CDw52, Cambridge pathology 1 antigen, Epididymal secretory protein E5, Human epididymis-specific protein 5 (He5), HE5
细胞定位

Cell membrane
Accession

P31358
克隆号

S-R431
抗体类型

Mouse mAb
抗体同种型

IgG1,k
反应种属 ?

Hu
纯化方式

Protein G
浓度

2 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20°C as supplied
应用

FCM

ICC ?
稀释度

应用稀释度

FCM 1:200
ICC 1:50

应用	稀释度
FCM	1:200
ICC	1:50

背景介绍

CD52 protein is a member of an unnamed short-chain GPI-anchored glycoprotein family in humans, which also includes human CD24 antigen and its homologues. CD52 protein is widely distributed and can be found on lymphocytes, monocytes, eosinophils, and dendritic cells derived from monocytes in the hematopoietic system, with a high density of up to 5×10^5 molecules per lymphocyte. In addition, CD52 antigen is also expressed to varying degrees on many lymphocytic malignancies and some acute myeloid leukemia cells. The CD52 protein may also play a role in the transport and targeting of carbohydrates, indicating that it participates in cell processes related to carbohydrate transport.

流式分析
- Flow cytometric analysis of human PBMC (human peripheral blood mononuclear cell) labelling CD52 antibody at 1/200 (1 μg) dilution (Right) compared with a Mouse monoclonal IgG isotype control (Left). Goat Anti - Mouse IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD3 - Alexa Fluor® 647 separately. Gated on total viable cells.
免疫细胞化学
- ICC shows positive staining in human PBMCs. Anti-CD52 antibody was used at 1/50 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).