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Claudin 3 Recombinant Rabbit mAb (S-819-131)

Clostridium perfringens enterotoxin receptor 2 (CPE-R 2,CPE-receptor 2),Rat ventral prostate.1 protein homolog (hRVP1),CLDN3,C7orf1,CPETR2

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0684

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
分子别名

Clostridium perfringens enterotoxin receptor 2 (CPE-R 2; CPE-receptor 2), Rat ventral prostate.1 protein homolog (hRVP1), CLDN3, C7orf1, CPETR2
免疫原

Synthetic Peptide
细胞定位

Cell membrane
Accession

O15551
克隆号

S-819-131
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu
纯化方式

Protein A
浓度

2 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

WB

IP
稀释度

应用稀释度

WB 1:5000
IHC-P 1:200
IP 1:200

应用	稀释度
WB	1:5000
IHC-P	1:200
IP	1:200

背景介绍

Claudin 3 protein is a tight junction protein with a molecular weight of approximately 22 kDa. Tight junctions are important membrane-associated complexes between adjacent cells, and Claudin proteins form the main backbone of these structures. Along with other Claudin proteins, Claudin-3 protein stabilizes the structure of intercellular junctions by interacting with each other. It helps maintain the stability of cell gaps and facilitates transmembrane transport/enrichment functions. Claudin-3 protein can also alter the permeability of intercellular junctions, controlling the passage of substances. Claudin-3 protein is often overexpressed in certain cancers, such as ovarian cancer and breast cancer. This increased expression level is associated with the enhancement of tumor cell invasiveness and vitality. Therefore, Claudin-3 may become a target for the treatment of prostate cancer and other malignancies. In cell susceptibility and demyelinating anatomical phenotypes, inhibition of Claudin-3 expression is associated with neurotoxicity.

免疫印迹
- WB result of Claudin 3 Rabbit mAb
  Primary antibody: Claudin 3 Rabbit mAb at 1/5000 dilution
  Lane 1: U-87MG whole cell lysate 20 µg
  Lane 2: MCF7 whole cell lysate 20 µg
  Lane 3: T-47D whole cell lysate 20 µg
  Lane 4: HT-29 whole cell lysate 20 µg
  Negative control: U-87MG whole cell lysate
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 23 kDa
  Observed MW: 20 kDa
免疫沉淀
- Claudin 3 Rabbit mAb at 1/200 dilution (1 µg) immunoprecipitating Claudin 3 in 0.4 mg MCF7 whole cell lysate.
  Western blot was performed on the immunoprecipitate using Claudin 3 Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/1000 dilution.
  Lane 1: MCF7 whole cell lysate 20 µg (Input)
  Lane 2: Claudin 3 Rabbit mAb IP in MCF7 whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in MCF7 whole cell lysate
  Predicted MW: 23 kDa
  Observed MW: 20 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human colon. Anti- Claudin 3 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human tonsil. Anti- Claudin 3 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human colon cancer. Anti- Claudin 3 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.