DCTN2 Recombinant Rabbit mAb (S-1082-7)

Dynactin subunit 2,50 kDa dynein-associated polypeptide,Dynactin complex 50 kDa subunit (DCTN-50),p50 dynamitin,DCTN50

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0683

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
分子别名

Dynactin subunit 2, 50 kDa dynein-associated polypeptide, Dynactin complex 50 kDa subunit (DCTN-50), p50 dynamitin, DCTN50
免疫原

Synthetic Peptide
细胞定位

Cytoplasm, Cytoskeleton
Accession

Q13561
克隆号

S-1082-7
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
预测反应种属
(反应种属缩写表)

Pg, Xe, Bv, Av
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICC ?

WB

IP
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms, Rt
ICC 1:500 Hu
IP 1:50 Hu

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms, Rt
ICC	1:500	Hu
IP	1:50	Hu

背景介绍

DCTN2, also known as Dynactin 2, is an important protein involved in cellular transport. DCTN2 is a component of the Dynactin complex, which is associated with the microtubule system in cells. It serves as a bridge between the Dynactin complex and Dynein, a protein responsible for transporting cellular organelles, RNA, and proteins along microtubules. The Dynactin complex, which includes DCTN2, is essential for various cellular functions such as the transport of the endoplasmic reticulum to the Golgi apparatus, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. DCTN2 plays a crucial role in the positioning and transport of organelles within the cell. By interacting with other components, it regulates the normal functioning of the cell. DCTN2 is also involved in the formation and maintenance of flagella, which are cellular protrusions responsible for cell movement and environmental sensing.

免疫印迹
- WB result of DCTN2 Rabbit mAb
  Primary antibody: DCTN2 Rabbit mAb at 1/1000 dilution
  Lane 1: MCF7 whole cell lysate 20 µg
  Lane 2: A375 whole cell lysate 20 µg
  Lane 3: Saos-2 whole cell lysate 20 µg
  Lane 4: HeLa whole cell lysate l0 µg
  Lane 5: K562 whole cell lysate 20 µg
  Lane 6: SH-SY5Y whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 44 kDa
  Observed MW: 48 kDa
- WB result of DCTN2 Rabbit mAb
  Primary antibody: DCTN2 Rabbit mAb at 1/1000 dilution
  Lane 1: mouse brain lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 44 kDa
  Observed MW: 48 kDa
- WB result of DCTN2 Rabbit mAb
  Primary antibody: DCTN2 Rabbit mAb at 1/1000 dilution
  Lane 1: rat brain lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 44 kDa
  Observed MW: 48 kDa
免疫沉淀
- DCTN2 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating DCTN2 in 0.4 mg HeLa whole cell lysate.
  Western blot was performed on the immunoprecipitate using DCTN2 Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/1000 dilution.
  Lane 1: HeLa whole cell lysate 20 µg (Input)
  Lane 2: DCTN2 Rabbit mAb IP in HeLa whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in HeLa whole cell lysate
  Predicted MW: 44 kDa
  Observed MW: 48 kDa
免疫细胞化学
- ICC shows positive staining in HeLa cells. Anti-DCTN2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).