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Glutathione Peroxidase 4 Recombinant Rabbit mAb (S-965-24)

Phospholipid hydroperoxide glutathione peroxidase GPX4,PHGPx,GPx-4,GSHPx-4,GPX4

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B0679
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产品介绍 FAQs 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 分子别名

    Phospholipid hydroperoxide glutathione peroxidase GPX4, PHGPx, GPx-4, GSHPx-4, GPX4

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Cytoplasm

  • Accession

    P36969, O70325

  • 克隆号

    S-965-24

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 反应种属 ?

    Hu, Ms, Rt

  • 预测反应种属
    (反应种属缩写表)

    Pr, Mq, Pg

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    WB

  • 稀释度

    应用 稀释度
    WB 1:1000
背景介绍

  • Glutathione peroxidase 4 (GPX4) is an enzyme that plays a crucial role in cellular homeostasis and protection against oxidative stress. It is a lipid repair enzyme that functions to detoxify lipid hydroperoxides, which are harmful reactive oxygen species (ROS) generated during oxidative stress. It uses glutathione (GSH) as a cofactor to reduce lipid hydroperoxides to their corresponding alcohols, thereby preventing lipid peroxidation and subsequent cell damage. GPX4 plays a key role in preventing ferroptosis, a type of cell death characterized by iron-dependent accumulation of lipid ROS. Ferroptosis occurs when GPX4 activity is impaired, leading to uncontrolled lipid peroxidation and cell death. GPX4 has been also implicated in a variety of biological processes, including cancer development, neurodegeneration, and organ damage.

  • 免疫印迹

    • WB result of Glutathione Peroxidase 4 Rabbit mAb
      Primary antibody: Glutathione Peroxidase 4 Rabbit mAb at 1/1000 dilution
      Lane 1: KATO III whole cell lysate 20 µg
      Lane 2: HeLa whole cell lysate 20 µg
      Lane 3: HepG2 whole cell lysate 20 µg
      Lane 4: 293T whole cell lysate 20 µg
      Lane 5: Jurkat whole cell lysate 20 µg
      Lane 6: U937 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 22 kDa
      Observed MW: 15 kDa

    • WB result of Glutathione Peroxidase 4 Rabbit mAb
      Primary antibody: Glutathione Peroxidase 4 Rabbit mAb at 1/1000 dilution
      Lane 1: mouse heart lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 22 kDa
      Observed MW: 15 kDa

    • WB result of Glutathione Peroxidase 4 Rabbit mAb
      Primary antibody: Glutathione Peroxidase 4 Rabbit mAb at 1/1000 dilution
      Lane 1: rat heart lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 22 kDa
      Observed MW: 15 kDa

FAQs

斯达特公司的抗体，可以回收利用几次？

我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定

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