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Mouse mAb IgG2a, κ Isotype Control (S-844-17)

货号： S0B0678

Datasheet COA
  • 价格： ￥500
  • 规格：
  • 数量：
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产品介绍 评论(0)

产品规格

  • 宿主来源

    Mouse

  • 免疫原

    Recombinant Protein

  • 克隆号

    S-844-17

  • 抗体类型

    Mouse mAb

  • 抗体同种型

    IgG2a,k

  • 应用

    IHC-P, FCM

  • 纯化方式

    Protein A

  • 浓度

    0.2 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 稀释度

    [{"application": "IHC-P", "dilution": "", "species": ""}, {"application": "ICFCM", "dilution": "", "species": ""}, {"application": "FCM", "dilution": "", "species": ""}]
背景介绍

  • Isotype control antibodies, to estimate the nonspecific binding of target. Use at concentrations comparable to those of the specific antibody of interest.

  • 流式分析

    • Flow cytometric analysis of Jurkat (Human T cell leukemia T lymphocyte) labeling IgG2a, κ Isotype Control at 1/20 (1 μg) dilution (Red) compared with a Mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Mouse IgG Alexa Fluor® 488 was used as the secondary antibody.

    • Flow cytometric analysis of Raji (Human Burkitt's lymphoma B lymphocyte) labeling IgG2a, κ Isotype Control at 1/20 (1 μg) dilution (Red) compared with a Mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Mouse IgG Alexa Fluor® 488 was used as the secondary antibody.

    • Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Mouse embryonic fibroblast) labelling IgG2a, κ Isotype Control at 1/20 (1 μg) dilution (Left) / (Red) compared with α-tubulin antibody (Right) / (Red), Mouse monoclonal IgG Isotype Control (Left) / (Black) compared with IgG2a, κ Isotype Control (Right) / (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Mouse IgG Alexa Fluor® 488 was used as the secondary antibody.

  • 免疫组化

    • IHC shows negative staining in paraffin-embedded human kidney. Mouse mAb IgG2a, κ Isotype Control was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows negative staining in paraffin-embedded human tonsil. Mouse mAb IgG2a, κ Isotype Control was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows negative staining in paraffin-embedded mouse liver. Mouse mAb IgG2a, κ Isotype Control was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows negative staining in paraffin-embedded rat spleen. Mouse mAb IgG2a, κ Isotype Control was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

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