HMGB1 Rabbit polyclonal antibody

High mobility group protein B1,High mobility group protein 1 (HMG-1),HMG1

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0673

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 引用文献(1) 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

HMGB1
分子别名

High mobility group protein B1, High mobility group protein 1 (HMG-1), HMG1
免疫原

Synthetic Peptide
细胞定位

Nucleus
Accession

P09429
抗体类型

Polyclonal antibody
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
预测反应种属
(反应种属缩写表)

Bv, Bb, Pg, Hm
纯化方式

Immunogen Affinity
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20°C as supplied
应用

ICFCM

ICC ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms, Rt
ICC 1:500 Hu
ICFCM 1:500 Hu

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms, Rt
ICC	1:500	Hu
ICFCM	1:500	Hu

背景介绍

HMGB1 protein, or High Mobility Group Box 1 protein, is a highly conserved nuclear protein found widely in mammalian cells. HMGB1 is prevalent in various tissues such as lymphatic tissue, brain, liver, lung, heart, spleen, and kidney. In most tissues, it primarily resides in the nucleus, but in certain tissues like the liver and brain, it can also be found in the cytoplasm. Within the nucleus, HMGB1 interacts with ribosomes, transcription factors, and other components, participating in maintaining nucleosome structure and regulating gene expression. It can also modulate gene transcription by facilitating nucleosome sliding. Outside the cell, HMGB1 serves as a late-phase inflammatory mediator, involving inflammation, immune responses, tissue repair, and regeneration. It mediates these biological effects by interacting with various receptors, such as Toll-like receptor 4 and the receptor for advanced glycation end products. HMGB1 plays a crucial role in systemic inflammatory response syndrome, such as sepsis. Sepsis is a common complication of severe trauma, burns, and other diseases, and HMGB1 is a key inflammatory mediator in this condition.

免疫印迹
- WB result of HMGB1 Rabbit pAb
  Primary antibody: HMGB1 Rabbit pAb at 1/1000 dilution
  Lane 1: HeLa whole cell lysate 20 µg
  Lane 2: Jurkat whole cell lysate 20 µg
  Lane 3: A431 whole cell lysate 20 µg
  Lane 4: HepG2 whole cell lysate 20 µg
  Lane 5: SK-BR-3 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 25 kDa
  Observed MW: 25 kDa
- WB result of HMGB1 Rabbit pAb
  Primary antibody: HMGB1 Rabbit pAb at 1/1000 dilution
  Lane 1: NIH/3T3 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 25 kDa
  Observed MW: 25 kDa
- WB result of HMGB1 Rabbit pAb
  Primary antibody: HMGB1 Rabbit pAb at 1/1000 dilution
  Lane 1: PC-12 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 25 kDa
  Observed MW: 25 kDa
流式分析
- Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) labelling HMGB1 antibody at 1/500 dilution (0.1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫细胞化学
- ICC shows positive staining in HeLa cells. Anti-HMGB1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).