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宿主来源
Rabbit分子别名
Mitochondrial Carbamoyl-phosphate synthase [ammonia], Carbamoyl-phosphate synthetase I (CPSase I)免疫原
Synthetic Peptide细胞定位
MitochondrionAccession
P31327克隆号
S-674-9抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ICFCM
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 WB 1:1000 IHC-P 1:500 ICC 1:50 ICFCM 1:50
The CPS1 protein, also known as carbamoyl phosphate synthetase I, plays a crucial role in the urea cycle. The CPS1 protein has a molecular weight of 163kDa and is primarily located in the mitochondria of the liver. It is a key enzyme in the urea cycle, responsible for synthesizing urea from ammonia and CO2 in the body. This process is essential for eliminating excess ammonia in the body, making it crucial for ammonia detoxification in animals. Mutations in the CPS1 can lead to carbamoyl phosphate synthetase I deficiency (CPSID), a rare autosomal recessive genetic disorder that causes abnormalities in ammonia metabolism. This condition triggers a genetic metabolic disease characterized by hyperammonemia. Studies have shown that the CPS1 is closely related to various malignancies such as liver cancer, lung cancer, and colorectal cancer. It may affect the occurrence and development of tumors through multiple signal transduction pathways or by influencing pyrimidine synthesis.
免疫印迹
WB result of CPS1 Rabbit mAb
Primary antibody: CPS1 Rabbit mAb at 1/1000 dilution
Lane 1: U-2 OS whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 10 µg
Lane 3: SK-BR-3 whole cell lysate 20 µg
Negative control: U-2 OS whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 165 kDa
Observed MW: 165 kDa
流式分析
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) labelling CPS1 antibody at 1/50 dilution (1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫组化
IHC shows positive staining in paraffin-embedded human liver. Anti-CPS1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-CPS1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti-CPS1 antibody was used at 1/50 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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