RPS3A Rabbit polyclonal antibody

Small ribosomal subunit protein eS1,40S ribosomal protein S3a,v-fos transformation effector protein (Fte-1),FTE1,MFTL

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0666

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
分子别名

Small ribosomal subunit protein eS1, 40S ribosomal protein S3a, v-fos transformation effector protein (Fte-1), FTE1, MFTL
免疫原

Synthetic Peptide
细胞定位

Cytoplasm
Accession

P61247
抗体类型

Polyclonal antibody
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
预测反应种属
(反应种属缩写表)

Av, Rb, Pr, Sn, Mq, Ct
纯化方式

Immunogen Affinity
浓度

0.1 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

WB

IP
稀释度

应用稀释度推荐种属

WB 1:2000 Hu, Ms, Rt
IHC-P 1:100 Hu, Ms, Rt
IP 1:25 Hu

应用	稀释度	推荐种属
WB	1:2000	Hu, Ms, Rt
IHC-P	1:100	Hu, Ms, Rt
IP	1:25	Hu

背景介绍

RPS3A, also known as 40S ribosomal protein S3a, is a protein that in humans is encoded by the RPS3A gene. It plays a role in ribosomal structure formation and protein binding, participating in protein biosynthesis. Recently, researchers found that the NAL21 gene in rice, which encodes RPS3A, regulates leaf width by modulating the translation levels of growth factor and leaf lateral expansion regulators. This study suggests that appropriate translation regulation of key transcription factors during leaf development is essential for maintaining normal leaf morphology. Moreover, RPS3A has been associated with certain diseases, such as spherocytosis types 1 and 4. It is also involved in pathways related to viral mRNA translation and metabolism.

免疫印迹
- WB result of RPS3A Rabbit pAb
  Primary antibody: RPS3A Rabbit pAb at 1/2000 dilution
  Lane 1: HepG2 whole cell lysate 20 µg
  Lane 2: HeLa whole cell lysate 20 µg
  Lane 3: 293T whole cell lysate 20 µg
  Lane 4: Jurkat whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 30 kDa
  Observed MW: 30 kDa
- WB result of RPS3A Rabbit pAb
  Primary antibody: RPS3A Rabbit pAb at 1/2000 dilution
  Lane 1: NIH/3T3 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 30 kDa
  Observed MW: 30 kDa
- WB result of RPS3A Rabbit pAb
  Primary antibody: RPS3A Rabbit pAb at 1/2000 dilution
  Lane 1: PC-12 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 30 kDa
  Observed MW: 35 kDa
免疫沉淀
- RPS3A Rabbit pAb at 1/25 dilution (0.4 µg) immunoprecipitating RPS3A in 0.4 mg HeLa whole cell lysate.
  Western blot was performed on the immunoprecipitate using RPS3A Rabbit pAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/1000 dilution.
  Lane 1: HeLa whole cell lysate 20 µg (Input)
  Lane 2: RPS3A Rabbit pAb IP in HeLa whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in HeLa whole cell lysate
  Predicted MW: 30 kDa
  Observed MW: 30 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti- RPS3A antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti- RPS3A antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti- RPS3A antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti- RPS3A antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.