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产品规格
宿主来源
Mouse分子别名
T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e细胞定位
Cell membraneAccession
P22646克隆号
145-2C11抗体类型
Recombinant mAb抗体同种型
IgG1,k应用
in vitro T cell stimulation/activation, in vivo T cell depletion, FCM反应种属 ?
Ms纯化方式
Protein G浓度
5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS pH7.4, containing no preservative
储存条件
2 to 8 °C for 2 weeks under sterile conditions;
-20 °C for 3 months under sterile conditions;
-80 °C for 24 months under sterile conditions.
Please avoid repeated freeze-thaw cycles.
稀释度
| 应用 | 稀释度 |
|---|---|
| FCM | 1:50000 |
流式分析
Flow cytometric analysis of C57BL/6 mouse splenocytes labeling CD3ε at 1/50000 (0.01 μg) dilution / (Right panel) compared with a Mouse IgG, Isotype Control / (Left panel). Goat Anti - Mouse IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD19 - Alexa Fluor® 647 separately. Gated on total viable cells.
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