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Invivo anti-mouse CD8α Recombinant mAb

T-cell surface glycoprotein CD8 alpha chain,T-cell surface glycoprotein Lyt-2,Lyt-2,Lyt2

价格 ￥700.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B0660
规格
数量
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产品介绍 引用文献(2) 评论(0)

产品规格

  • 宿主来源

    Rat

  • 分子别名

    T-cell surface glycoprotein CD8 alpha chain, T-cell surface glycoprotein Lyt-2, Lyt-2, Lyt2

  • 细胞定位

    Cell membrane

  • Accession

    P01731

  • 克隆号

    2.43

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    Rat IgG2b, κ

  • 反应种属 ?

    Ms

  • 纯化方式

    Protein G

  • 浓度

    Lot specific* (generally 5 to 10 mg/ml)*

  • 纯度

    >95% (Determined by SDS-PAGE)

  • 内毒素含量

    <2EU/mg

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS pH7.4, containing no preservative

  • 储存条件

    2 to 8 °C for 2 weeks under sterile conditions;

    -20 °C for 3 months under sterile conditions;

    -80 °C for 24 months under sterile conditions.

    Please avoid repeated freeze-thaw cycles.

  • 应用

    in vivo CD8+ T cell depletion ,
    FCM ,
    WB

  • 稀释度

    应用 稀释度
    WB 1:50
    FCM 1:50000
背景介绍

  • CD8a, also known as CD8α, is a molecular marker on the surface of T lymphocytes. It belongs to the family of leukocyte differentiation antigens and is primarily expressed on the surface of cytotoxic T lymphocytes (CTLs). CD8a plays a crucial role in immune responses related to cytotoxicity and local inflammation. It has the ability to bind to MHC class I molecules. The expression level of CD8 molecules undergoes changes during various stages of T cell differentiation, development, and in response to T cell stimuli. Additionally, CD8a is closely associated with autoimmune monitoring, humoral immune responses, and transplant reactions. For instance, CD8a can serve as a monitoring indicator for certain autoimmune diseases and plays a significant role in immunotherapy for anti-tumor and anti-viral treatments.

  • 免疫印迹

    • WB result of Invivo anti-mouse CD8α Rat mAb
      Primary antibody: Invivo anti-mouse CD8α Rat mAb at 1/50 dilution
      Lane 1: Mouse CD8, His tag (S0A1018) 1 µg
      Lane 2: Mouse CD8, His tag (S0A1018) 0.5 µg
      Secondary antibody: Goat Anti-rat IgG, (H+L), HRP conjugated at 1/1000 dilution
      Predicted MW: 35 kDa
      Observed MW: 25~35 kDa

  • 流式分析

    • Flow cytometric analysis of C57BL/6 mouse splenocytes labeling CD8α at 1/50000 (0.01 μg) dilution / (Right panel) compared with a Rat IgG, Isotype Control / (Left panel). Goat Anti - Rat IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD3 - Alexa Fluor® 647 separately. Gated on total viable cells.

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