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宿主来源
Rabbit分子别名
Integrin alpha-6, CD49 antigen-like family member F, VLA-6, ITGA6免疫原
Synthetic Peptide细胞定位
Cell membraneAccession
P23229克隆号
S-1019-73抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt IHC-P 1:100 Hu
CD49f protein, also known as integrin-6 (ITGA6) or integrin alpha-6 chain protein, is a type I transmembrane glycoprotein belonging to the α-subfamily. CD49f may have two distinct cytoplasmic domains resulting from alternative splicing variants of mRNA, α6A and α6B, which exhibit different stem-like functions. Among them, the α6B variant is closely related to the cancer stem cell (CSC) phenotype. CD49f plays a crucial role in cell adhesion to the extracellular matrix. It typically forms heterodimers with integrin β1 and β4, serving as a receptor for various laminin proteins. Research has shown that the heterodimerization of CD49f interacts with signaling pathways, inducing stem-like and invasive characteristics in breast cancer cells. Moreover, CD49f is not only a stemness-related gene that can serve as a surface marker for various stem cells, such as embryonic stem cells, neural stem cells, and hematopoietic stem cells, but also plays a significant role in maintaining stem cell self-renewal and stemness through its interaction with ECM components (e.g., laminin) in the microenvironment. Previous studies have also found that the adhesion molecule CD49f is differentially expressed in fetal and adult bone marrow-derived mesenchymal stem cells (BMSCs), and the interaction between stem cells and their supporting cells or ECM in the microenvironment mediated by CD49f is crucial for maintaining stemness.
免疫印迹
WB result of CD49f Rabbit mAb
Primary antibody: CD49f Rabbit mAb at 1/1000 dilution
Lane 1: SW480 whole cell lysate 20 µg
Lane 2: A549 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 127 kDa
Observed MW: 127 kDaWB result of CD49f Rabbit mAb
Primary antibody: CD49f Rabbit mAb at 1/1000 dilution
Lane 1: mouse lung lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 127 kDa
Observed MW: 127 kDaWB result of CD49f Rabbit mAb
Primary antibody: CD49f Rabbit mAb at 1/1000 dilution
Lane 1: rat stomach lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 127 kDa
Observed MW: 127 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human kidney. Anti-CD49f antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human placenta. Anti-CD49f antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human stomach. Anti-CD49f antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-CD49f antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-CD49f antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-CD49f antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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