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ALOX15 Recombinant Rabbit mAb (S-669-56)

Polyunsaturated fatty acid lipoxygenase ALOX15,12/15-lipoxygenase,leukocyte-type Arachidonate 12-lipoxygenase (12-LOX),Arachidonate 15-lipoxygenase (15-LOX,15-LOX-1),Arachidonate omega-6 lipoxygenase,Hepoxilin A3 synthase Alox15,Linoleate 13S-lipoxygenase

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0656

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
分子别名

Polyunsaturated fatty acid lipoxygenase ALOX15, 12/15-lipoxygenase, leukocyte-type Arachidonate 12-lipoxygenase (12-LOX), Arachidonate 15-lipoxygenase (15-LOX; 15-LOX-1), Arachidonate omega-6 lipoxygenase, Hepoxilin A3 synthase Alox15, Linoleate 13S-lipoxygenase
免疫原

Synthetic Peptide
细胞定位

Cell membrane, Cytoplasm
Accession

P16050
克隆号

S-669-56
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu
预测反应种属
(反应种属缩写表)

Or
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

WB
稀释度

应用稀释度

WB 1:1000
IHC-P 1:1000

应用	稀释度
WB	1:1000
IHC-P	1:1000

背景介绍

The ALOX15 protein is encoded by the Alox15 gene and functions as a non-heme iron-containing dioxygenase that catalyzes and esterifies the peroxidation of polyunsaturated fatty acids, producing a series of bioactive lipid intermediates. This enzyme inserts a peroxy group at the C12 or C15 position of arachidonic acid esters, generating 12-HPETE and 15-HPETE, and further metabolizes 12-HPETE to produce pro-inflammatory factors such as hepoxilins. Additionally, Alox15 can also peroxide linoleic acid esters to 13-HPODE and participate in the oxidation of DHA to produce specific pro-resolving mediators (SPMs), such as resolvin D5 and (7S,14S)-diHPDHA, which actively down-regulate immune responses and have anti-platelet aggregation properties. Beyond its biochemical functions, the ALOX15 protein also has important applications in the medical field. Studies have shown that recombinant ALOX15 protein can increase the level of interferon IFNβ in the body, effectively blocking pathogenic infections, independent of its enzymatic activity. Molecular mechanism studies have confirmed that the ALOX15 protein promotes the K63 deubiquitination modification of the mitochondrial protein MAVS, increasing the aggregation and activation of MAVS, and subsequently inducing the secretion of IFNβ. Therefore, the recombinant ALOX15 protein can be applied to enhance immunity and prevent and treat pathogenic infections and their complications, including influenza A and B viruses, novel coronavirus, Mycobacterium tuberculosis, Chlamydia trachomatis, and others.

免疫印迹
- WB result of ALOX15 Rabbit mAb
  Primary antibody: ALOX15 Rabbit mAb at 1/1000 dilution
  Lane 1: HeLa whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 75 kDa
  Observed MW: 70 kDa
  (This blot was developed with high sensitivity substrate)
免疫组化
- IHC shows positive staining in paraffin-embedded human colon. Anti-ALOX15 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human spleen. Anti-ALOX15 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human tonsil. Anti-ALOX15 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-ALOX15 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.