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PARP1 Recombinant Rabbit mAb (S-220-179)

Poly [ADP-ribose] polymerase 1,ADP-ribosyltransferase diphtheria toxin-like 1 (ARTD1),DNA ADP-ribosyltransferase PARP1,NAD(+) ADP-ribosyltransferase 1 (ADPRT 1),Poly[ADP-ribose] synthase 1,Protein poly-ADP-ribosyltransferase PARP1,ADPRT,PPOL

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B0650
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产品介绍 FAQs 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 分子别名

    Poly [ADP-ribose] polymerase 1, ADP-ribosyltransferase diphtheria toxin-like 1 (ARTD1), DNA ADP-ribosyltransferase PARP1, NAD(+) ADP-ribosyltransferase 1 (ADPRT 1), Poly[ADP-ribose] synthase 1, Protein poly-ADP-ribosyltransferase PARP1, ADPRT, PPOL

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Nucleus, Cytoplasm

  • Accession

    P09874

  • 克隆号

    S-220-179

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 反应种属 ?

    Hu, Ms, Rt

  • 预测反应种属
    (反应种属缩写表)

    Bv, Zf, Hm, Av

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    IHC-P ? ,
    WB

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:1000 Hu
    IHC-P 1:250 Hu, Ms, Rt
背景介绍

  • PARP1, short for poly (ADP-ribose) polymerase 1, is an enzyme that plays a pivotal role in DNA repair, maintenance of genomic integrity, and regulation of various metabolic and signaling processes. Discovered over 50 years ago, PARP1 has gradually been recognized for its critical functions in cellular biology. PARP1 catalyzes the transfer of ADP-ribose residues from NAD+ to target substrates, resulting in the formation of a poly (ADP-ribose) (PAR) chain. This PAR modification, known as PARylation, facilitates PARP1's involvement in DNA repair, stabilization of DNA replication forks, and modulation of chromatin structure. The primary functions of PARP1 can be described in four aspects: DNA base excision repair (BER), suppression of transcription from damaged DNA, depletion of cellular energy leading to apoptosis, and participation in transcriptional regulation of certain genes. The loss of PARP1 function has been associated with increased risk of cancers such as breast and lung cancer.

  • 免疫印迹

    • WB result of PARP1 Rabbit mAb
      Primary antibody: PARP1 Rabbit mAb at 1/1000 dilution
      Lane 1: untreated HeLa whole cell lysate 20 µg
      Lane 2: HeLa treated with 1 μM Staurosporine for 3 hours whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 113 kDa
      Observed MW: 120 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human testis. Anti-PARP1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human tonsil. Anti-PARP1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-PARP1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human ovarian carcinoma. Anti-PARP1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse testis. Anti-PARP1 mouse testis antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat testis. Anti-PARP1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

FAQs

斯达特公司的抗体，可以回收利用几次？

我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定

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