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MyD88 Recombinant Rabbit mAb (S-788-32)

Myeloid differentiation primary response protein MyD88

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B0647
规格
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产品介绍

产品介绍 FAQs 引用文献(1) 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 分子别名

    Myeloid differentiation primary response protein MyD88

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Nucleus, Cytoplasm

  • Accession

    Q99836

  • 克隆号

    S-788-32

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 反应种属 ?

    Hu, Ms, Rt

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    WB

    IP

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:1000 Hu, Ms, Rt
    IP 1:50 Hu
背景介绍

  • MyD88, also known as myeloid differentiation primary response gene 88, is a protein encoded by the MyD88 gene. It belongs to the Toll/IL-1R family and the death domain family, and is essentially a cytosolic soluble protein. The structure of MyD88 protein includes an N-terminal death domain (DD), a middle structural domain, and a C-terminal Toll/interleukin-1 receptor (TIR) domain. The DD region can interact with other proteins possessing DD to form homodimers or heterodimers, which further induce the activation of c-Jun N-terminal kinase (JNK) or nuclear factor-κB (NF-κB). It is also associated with TLR-mediated apoptosis. On the other hand, the TIR domain can bind to other TIR domains, mediating signal transduction. MyD88 plays a crucial role in the immune system, serving as a key adaptor molecule in the Toll-like receptor (TLR) signaling pathway. Primarily located in the cytoplasm, it functions as a pure adapter connecting IL-1R1 to downstream IRAK kinases. By recognizing pathogens, MyD88 induces the production of proinflammatory cytokines and upregulation of costimulatory molecules, triggering rapid activation of the innate immune response. Additionally, MyD88 is involved in regulating genes related to immunity, inflammation, and cell survival.

  • 免疫印迹

    • WB result of MyD88 Rabbit mAb
      Primary antibody: MyD88 Rabbit mAb at 1/1000 dilution
      Lane 1: A549 whole cell lysate 20 µg
      Lane 2: K562 whole cell lysate 20 µg
      Lane 3: Raji whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 33 kDa
      Observed MW: 35 kDa

    • WB result of MyD88 Rabbit mAb
      Primary antibody: MyD88 Rabbit mAb at 1/1000 dilution
      Lane 1: mouse spleen lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 33 kDa
      Observed MW: 33 kDa

    • WB result of MyD88 Rabbit mAb
      Primary antibody: MyD88 Rabbit mAb at 1/1000 dilution
      Lane 1: rat spleen lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 33 kDa
      Observed MW: 33 kDa

  • 免疫沉淀

    • MyD88 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating MyD88 in 0.4 mg K562 whole cell lysate.
      Western blot was performed on the immunoprecipitate using MyD88 Rabbit mAb at 1/1000 dilution.
      Secondary antibody (HRP) for IP was used at 1/1000 dilution.
      Lane 1: K562 whole cell lysate 20 µg (Input)
      Lane 2: MyD88 Rabbit mAb IP in K562 whole cell lysate
      Lane 3: Rabbit monoclonal IgG IP in K562 whole cell lysate
      Predicted MW: 33 kDa
      Observed MW: 35 kDa

FAQs

斯达特公司的抗体，可以回收利用几次？

我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定

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