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FABP7 Recombinant Rabbit mAb (S-762-114)

Brain fatty acid-binding protein,Brain lipid-binding protein (BLBP),Brain-type fatty acid-binding protein (B-FABP),Fatty acid-binding protein 7,Mammary-derived growth inhibitor related,BLBP,FABPB,MRG

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0646

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
分子别名

Brain fatty acid-binding protein, Brain lipid-binding protein (BLBP), Brain-type fatty acid-binding protein (B-FABP), Fatty acid-binding protein 7, Mammary-derived growth inhibitor related, BLBP, FABPB, MRG
免疫原

Recombinant Protein
细胞定位

Cytoplasm
Accession

O15540
克隆号

S-762-114
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

WB

IF ?
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms, Rt
IHC-P 1:200 Hu, Ms, Rt
IF 1:200-1:500 Ms, Rt

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms, Rt
IHC-P	1:200	Hu, Ms, Rt
IF	1:200-1:500	Ms, Rt

背景介绍

FABP7 protein is a highly expressed protein in the nervous systems of humans and animals. Structurally, it consists of 10 β-strand segments that form a three-dimensional structure enclosing a fatty acid binding pocket. It is mainly present in astrocytes and neural precursor cells in the adult nervous system. By binding to n-3 and n-9 polyunsaturated fatty acids (PUFAs), such as docosahexaenoic acid (DHA) and oleic acid, FABP7 regulates the metabolism of these fatty acids. FABP7 protein plays a crucial role in neural development, cell migration, and signal transduction. In the embryonic brain, it is essential for the maintenance and proliferation of neural stem progenitor cells and radial glial cells.

免疫印迹
- WB result of FABP7 Rabbit mAb
  Primary antibody: FABP7 Rabbit mAb at 1/1000 dilution
  Lane 1: A549 whole cell lysate 20 µg
  Lane 2: SK-MEL-28 whole cell lysate 40 µg
  Negative control: A549 whole cell lysate
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 15 kDa
  Observed MW: 14 kDa
- WB result of FABP7 Rabbit mAb
  Primary antibody: FABP7 Rabbit mAb at 1/1000 dilution
  Lane 1: mouse kidney lysate 20 µg
  Lane 2: mouse brain lysate 20 µg
  Lane 3: mouse cerebellum lysate 20 µg
  Negative control: mouse kidney lysate
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 15 kDa
  Observed MW: 14 kDa
- WB result of FABP7 Rabbit mAb
  Primary antibody: FABP7 Rabbit mAb at 1/1000 dilution
  Lane 1: rat kidney lysate 20 µg
  Lane 2: rat brain lysate 20 µg
  Lane 3: rat cerebellum lysate 20 µg
  Negative control: rat kidney lysate
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 15 kDa
  Observed MW: 14 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human cerebellum. Anti-FABP7 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-FABP7 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative control: IHC shows negative staining in paraffin-embedded human kidney. Anti-FABP7 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse cerebellum. Anti-FABP7 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-FABP7 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative control: IHC shows negative staining in paraffin-embedded mouse kidney. Anti-FABP7 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat cerebellum. Anti-FABP7 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative control: IHC shows negative staining in paraffin-embedded rat kidney. Anti-FABP7 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
- IF shows positive staining in paraffin-embedded mouse cerebellum. Anti-FABP7 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
- IF shows positive staining in paraffin-embedded rat cerebellum. Anti-FABP7 antibody was used at 1/200 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.