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Mouse细胞定位
Cytoplasm, Nucleus克隆号
S-R180抗体类型
Recombinant mAb抗体同种型
IgG2a反应种属 ?
Hu, Ms纯化方式
Protein A浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20°C as supplied
应用
ICC ?
稀释度
应用 稀释度 ICC 1:500
RNA:DNA hybrids serve as primers for DNA replication, regulate gene expression and are structural components of specific eukaryotic genomic elements, such as telomeres, centromeres and ribosomal DNA. RNA:DNA hybridization is also a crucial step in cell-specific processes, such as immunoglobulin class-switch recombination (CSR) in activated B cells and CRISPR/Cas9-mediated adaptive immunization in prokaryotic cells.
免疫细胞化学
ICC shows positive staining in NIH/3T3 cells. Anti-DNA:RNA hybrid antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in HeLa cells. Anti-DNA:RNA hybrid antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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