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COX2 Recombinant Rabbit mAb (S-870-18)

Prostaglandin G/H synthase 2,Cyclooxygenase-2,Glucocorticoid-regulated inflammatory cyclooxygenase,Gripghs,Macrophage activation-associated marker protein P71/73,PES-2,PHS II,Prostaglandin H2 synthase 2 (PGH synthase 2,PGHS-2),Prostaglandin-endoperoxide synthase 2,TIS10 protein,Ptgs2,Cox-2,Pghs-b,Tis10

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B0629
规格
数量
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产品介绍

产品介绍 FAQs 引用文献(1) 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 分子别名

    Prostaglandin G/H synthase 2, Cyclooxygenase-2, Glucocorticoid-regulated inflammatory cyclooxygenase, Gripghs, Macrophage activation-associated marker protein P71/73, PES-2, PHS II, Prostaglandin H2 synthase 2 (PGH synthase 2; PGHS-2), Prostaglandin-endoperoxide synthase 2, TIS10 protein, Ptgs2, Cox-2, Pghs-b, Tis10

  • 免疫原

    Recombinant Protein

  • 细胞定位

    Cytoplasm

  • Accession

    Q05769

  • 克隆号

    S-870-18

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 反应种属 ?

    Ms, Rt

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    IHC-P ?

    ICC ?

    WB

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:1000 Ms
    IHC-P 1:500 Ms, Rt
    ICC 1:500 Ms
背景介绍

  • COX-2 protein, also known as Cyclooxygenase-2 or PGHS-2, is a member of the cyclooxygenase superfamily. This recombinant protein plays a specific enzymatic role in the biological system, converting arachidonic acid (AA) into prostaglandin G2 and other peroxide substances, participating in the biosynthesis of prostaglandins. Under normal physiological conditions, most tissue cells do not express COX-2 protein. However, in pathological states such as inflammation and tumorigenesis, COX-2 protein expression is upregulated in response to proinflammatory mediators, including inflammatory stimuli, damage, mitogens, and carcinogens. This process involves the release of arachidonic acid from cell membrane phospholipids through the phospholipase A2 pathway, followed by the synthesis of inflammatory mediators such as prostaglandin E2 (PGE2) catalyzed by COX-2, participating in various physiological and pathological processes in the body. Moreover, the enzymatic activity of COX-2 protein is not limited to arachidonic acid. It can also act on dihomo-γ-linolenic acid (DGLA) and eicosapentaenoic acid (EPA), generating PGH1 and PGH3 as precursors of different series of prostaglandins.

  • 免疫印迹

    • WB result of Mouse COX2 Rabbit mAb
      Primary antibody: Mouse COX2 Rabbit mAb at 1/1000 dilution
      Lane 1: untreated RAW 264.7 whole cell lysate 20 µg
      Lane 2: RAW 264.7 treated with 1 µg/ml lipopolysaccharide for 6 hours whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 69 kDa
      Observed MW: 75 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded mouse colon. Anti- Mouse COX2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse liver. Anti- Mouse COX2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat colon. Anti- Mouse COX2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat liver. Anti- Mouse COX2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat kidney. Anti- Mouse COX2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫细胞化学

    • ICC analysis of RAW264.7 cells treated with lipopolysaccharide (LPS) (1 µg/ml,6h) (top panel) and RAW264.7 cells untreated with lipopolysaccharide (LPS) (1 µg/ml,6h) (below panel). Anti-Mouse COX2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

FAQs

斯达特公司的抗体，可以回收利用几次？

我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定

引用文献(1)

  • Oxylipins from n-6 and n-3 fatty acids modulate uterine decidualization

    Dan Zhu,Dezhi Ma, Mengyun Wu,Jiayao Sun,Tian He, Yuan Qin, Xin Wang,Bingrui Yang, Xiaocui Zhong, Shan-An Chan, Shufang Chang, Boris Novakovic, Sergey Tumanov, Silas Villas Bôas, Heng Zou, Yang Yang, Ting-Li Han

    Biol Reprod. 2024 Nov 20 .

    影响因子： 3

    货号：S0B0629产品名称：Rabbit Anti-Mouse COX2 Recombinant mAb (S-870-18)

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