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Mouse分子别名
Fibronectin receptor subunit beta, Glycoprotein IIa (GPIIA), VLA-4 subunit beta, ITGB1, FNRB, MDF2, MSK12免疫原
Recombinant Protein细胞定位
Cell membraneAccession
P05556克隆号
S-933-228抗体类型
Mouse mAb抗体同种型
IgG1,k反应种属 ?
Hu纯化方式
Protein G浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
FCM
ICC ?
WB
稀释度
应用 稀释度 WB 1:1000 ICC 1:100 FCM 1:200
CD29 is an integrin component that mediates adhesion and involves in homing to sites of inflammation. It expresses in fibroblasts, platelets, T cells, monocytes, granulocytes(low), mast cells, endothelial cells and myoepithelium, also other diverse cell types. It does not express in red blood cells and spermatogonia. It is a myoepithelial marker, although established markers (SMA, CD10, p63, S100, maspin, calponin, GFAP, smooth muscle myosin) are more commonly used.
免疫印迹
WB result of CD29 Mouse mAb
Primary antibody: CD29 Mouse mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: A-431 whole cell lysate 20 µg
Lane 3: A549 whole cell lysate 20 µg
Lane 4: U-87 MG whole cell lysate 20 µg
Lane 5: MCF7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 88 kDa
Observed MW: 110~140 kDa
(This blot was developed with high sensitivity substrate)
流式分析
Flow cytometric analysis of A549 (Human lung carcinoma epithelial cell) labelling CD29 antibody at 1/200 dilution (1 μg) / (Red) compared with a Mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Mouse IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫细胞化学
ICC shows positive staining in A549 cells. Anti-CD29 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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