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Runx1 Recombinant Rabbit mAb (S-959-64)

Runt-related transcription factor 1,Acute myeloid leukemia 1 protein,Core-binding factor subunit alpha-2 (CBF-alpha-2),Oncogene AML-1,Polyomavirus enhancer-binding protein 2 alpha B subunit (PEA2-alpha B,PEBP2-alpha B),SL3-3 enhancer factor 1 alpha B subunit,SL3/AKV core-binding factor alpha B subunit

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0621

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
分子别名

Runt-related transcription factor 1; Acute myeloid leukemia 1 protein; Core-binding factor subunit alpha-2 (CBF-alpha-2); Oncogene AML-1; Polyomavirus enhancer-binding protein 2 alpha B subunit (PEA2-alpha B; PEBP2-alpha B); SL3-3 enhancer factor 1 alpha B subunit; SL3/AKV core-binding factor alpha B subunit
免疫原

Synthetic Peptide
细胞定位

Nucleus
Accession

Q01196
克隆号

S-959-64
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICFCM

ChIP

ICC ?

WB

IP
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms, Rt
IP 1:50 Hu
ICC 1:500 Hu
ICFCM 1:5000 Hu
ChIP 1:20-1:50 Hu

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms, Rt
IP	1:50	Hu
ICC	1:500	Hu
ICFCM	1:5000	Hu
ChIP	1:20-1:50	Hu

背景介绍

Runx1 is a crucial transcription factor that binds to the core elements of many enhancers and promoters, regulating the expression of specific genes. This protein plays a pivotal role in cellular development, particularly in the development of hematopoietic stem cells. RUNX1 interacts with CBFβ (core-binding factor β) to form a set of transcription factors, and this interaction is essential in the development of neurons and hematopoietic stem cells. However, when CBFβ undergoes translocation, it can form a fusion protein CBFβ-SMMHC that abnormally binds tightly to RUNX1, participating in the pathogenesis of acute lymphocytic leukemia (ALL). Therefore, the RUNX1−CBFβ interaction is also considered an important target for cancer treatment. Furthermore, research on RUNX1 protein has revealed its association with a range of cancers, particularly leukemia. Chromosome translocations involving the RUNX1 gene have been clearly linked to several types of leukemia.

免疫印迹
- WB result of Runx1 Rabbit mAb
  Primary antibody: Runx1 Rabbit mAb at 1/1000 dilution
  Lane 1: HEK-293 whole cell lysate 20 µg
  Lane 2: Jurkat whole cell lysate 20 µg
  Lane 3: THP-1 whole cell lysate 20 µg
  Lane 4: MOLT-4 whole cell lysate 20 µg
  Lane 5: SW620 whole cell lysate 20 µg
  Weak expression: HEK-293 whole cell lysate
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 48 kDa
  Observed MW: 55 kDa
  Exposure time: 180 s
- WB result of Runx1 Rabbit mAb
  Primary antibody: Runx1 Rabbit mAb at 1/1000 dilution
  Lane 1: mouse thymus lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 48 kDa
  Observed MW: 55 kDa
  Exposure time: 120 s
- WB result of Runx1 Rabbit mAb
  Primary antibody: Runx1 Rabbit mAb at 1/1000 dilution
  Lane 1: rat thymus lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 48 kDa
  Observed MW: 55 kDa
  Exposure time: 120 s
流式分析
- Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized Jurkat (Human T cell leukemia T lymphocyte) labelling Runx1 antibody at 1/5000 dilution (0.01 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫沉淀
- Runx1 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Runx1 in 0.4 mg Jurkat whole cell lysate.
  Western blot was performed on the immunoprecipitate using Runx1 Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/1000 dilution.
  Lane 1: Jurkat whole cell lysate 10 µg (Input)
  Lane 2: Runx1 Rabbit mAb IP in Jurkat whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in Jurkat whole cell lysate
  Predicted MW: 48 kDa
  Observed MW: 55 kDa
  This blot was developed with high sensitivity substrate
免疫细胞化学
- ICC shows positive staining in Jurkat cells (top panel) and weak staining in HEK293 cells (below panel). Anti-RUNX1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ChIP
- Chromatin immunoprecipitation (ChIP) was performed on Jurkat cells cross - linked with 1% formaldehyde for 10 min, then chromatin was fragmented by sonication. Parallel reactions used Runx1 Recombinant Rabbit mAb (S-959-64) and Rabbit mAb IgG Isotype Control (SDT-R173) at 1:50 for immunoprecipitation.
  Post -immunoprecipitation, both samples were washed, eluted, and cross - links reversed. Purified DNA was analyzed by qPCR.
  qPCR showed the enrichment of LMO2, CD19 and SAT-α in
  Runx1 Recombinant Rabbit mAb (S-959-64)
  -immunoprecipitated sample.