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PSME1 Recombinant Rabbit mAb (S-732-14)

Proteasome activator complex subunit 1,11S regulator complex subunit alpha (REG-alpha),Activator of multicatalytic protease subunit 1,Interferon gamma up-regulated I-5111 protein (IGUP I-5111),Proteasome activator 28 subunit alpha (PA28a,PA28alpha)

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0620

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
分子别名

Proteasome activator complex subunit 1, 11S regulator complex subunit alpha (REG-alpha), Activator of multicatalytic protease subunit 1, Interferon gamma up-regulated I-5111 protein (IGUP I-5111), Proteasome activator 28 subunit alpha (PA28a; PA28alpha)
免疫原

Synthetic Peptide
细胞定位

Cytoplasm
Accession

Q06323
克隆号

S-732-14
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
预测反应种属
(反应种属缩写表)

Bv, Pg, Mq
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICFCM

IHC-P ?

ICC ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms, Rt
IHC-P 1:500 Hu, Ms, Rt
ICC 1:100 Hu
ICFCM 1:50 Hu

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms, Rt
IHC-P	1:500	Hu, Ms, Rt
ICC	1:100	Hu
ICFCM	1:50	Hu

背景介绍

Proteasome activator complex subunit 1 is a protein that in humans is encoded by the PSME1 gene. PSME1 is the alpha subunit of the 11S regulator, one of the two 11S subunits that is induced by gamma-interferon. The PSME1 protein plays a crucial role in the assembly of the immunoproteasome and is indispensable for effective antigen processing. As part of the PA28 activator complex, PSME1 collaborates with PSME2 to actively enhance the production of class I-binding peptides by altering the proteolytic cleavage pattern of the proteasome.

免疫印迹
- WB result of PSME1 Rabbit mAb
  Primary antibody: PSME1 Rabbit mAb at 1/1000 dilution
  Lane 1: Jurkat whole cell lysate 20 µg
  Lane 2: HeLa whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 29 kDa
  Observed MW: 25 kDa
- WB result of PSME1 Rabbit mAb
  Primary antibody: PSME1 Rabbit mAb at 1/1000 dilution
  Lane 1: RAW 264.7 whole cell lysate 20 µg
  Lane 2: Neuro-2a whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 29 kDa
  Observed MW: 25 kDa
- WB result of PSME1 Rabbit mAb
  Primary antibody: PSME1 Rabbit mAb at 1/1000 dilution
  Lane 1: C6 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 29 kDa
  Observed MW: 25 kDa
流式分析
- Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) labelling PSME1 antibody at 1/50 dilution (1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
- Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HepG2 (Human hepatocellular carcinoma epithelial cell) labelling PSME1 antibody at 1/50 dilution (1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫组化
- IHC shows positive staining in paraffin-embedded human colon. Anti-PSME1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human prostate. Anti-PSME1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-PSME1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human prostatic cancer. Anti-PSME1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse colon. Anti-PSME1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat stomach. Anti-PSME1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
- ICC shows positive staining in HeLa cells. Anti-PSME1 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).