MTAP Recombinant Rabbit mAb (S-813-23)

S-methyl-5'-thioadenosine phosphorylase,5'-methylthioadenosine phosphorylase,MTA phosphorylase,MTAPase,MSAP

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0610

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
分子别名

S-methyl-5'-thioadenosine phosphorylase, 5'-methylthioadenosine phosphorylase, MTA phosphorylase, MTAPase, MSAP
免疫原

Synthetic Peptide
细胞定位

Cytoplasm, Nucleus
Accession

Q13126
克隆号

S-813-23
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms, Rt
IHC-P 1:250 Hu

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms, Rt
IHC-P	1:250	Hu

背景介绍

S-methyl-5'-thioadenosine phosphorylase (MTAP) is an enzyme that plays a major role in polyamine metabolism and is important for the salvage of both adenine and methionine. It is responsible for the first step in this pathway, where it catalyzes the reversible phosphorylation of MTA to adenine and 5-methylthioribose-1-phosphate. This takes place after MTA is generated from S-adenosylmethionine.

免疫印迹
- WB result of MTAP Rabbit mAb
  Primary antibody: MTAP Rabbit mAb at 1/1000 dilution
  Lane 1: A549 whole cell lysate 20 µg
  Lane 2: MCF7 whole cell lysate 20 µg
  Lane 3: HeLa whole cell lysate 20 µg
  Lane 4: HEK-293 whole cell lysate 20 µg
  Lane 5: HT-29 whole cell lysate 20 µg
  Negative control: A549 whole cell lysate ; MCF7 whole cell lysate
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 31 kDa
  Observed MW: 28 kDa
  This blot was developed with high sensitivity substrate
- WB result of MTAP Rabbit mAb
  Primary antibody: MTAP Rabbit mAb at 1/1000 dilution
  Lane 1: RAW 264.7 whole cell lysate 20 µg
  Lane 2: NIH/3T3 whole cell lysate 20 µg
  Lane 3: mouse kidney lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 31 kDa
  Observed MW: 28 kDa
  This blot was developed with high sensitivity substrate
- WB result of MTAP Rabbit mAb
  Primary antibody: MTAP Rabbit mAb at 1/1000 dilution
  Lane 1: C6 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 31 kDa
  Observed MW: 28 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human colon. Anti-MTAP antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-MTAP antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.