分享产品介绍 评论(0)
宿主来源
Rabbit抗原名称
Lamin B2分子别名
LMNB2, LMN2免疫原
Synthetic Peptide细胞定位
Nucleus laminaAccession
Q03252克隆号
S-951-70抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt预测反应种属
(反应种属缩写表)Xe纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied.应用
ICFCM
IHC-P ?
ICC ?
WB
IP
IF ?
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:10000 Hu, Ms, Rt IHC-P 1:2000 Hu, Ms, Rt ICC 1:500 Hu, Ms ICFCM 1:500 Hu IF 1:500 Hu IP 1:50 Hu
Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. Lamin B2 is a protein that in humans is encoded by the LMNB2 gene. It is the second of two type B nuclear lamins, and it is associated with laminopathies.
免疫印迹
WB result of Lamin B2 Rabbit mAb
Primary antibody: Lamin B2 Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: Raji whole cell lysate 20 µg
Lane 4: HCT-116 whole cell lysate 20 µg
Lane 5: Jurkat whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 68 kDa
Observed MW: 70 kDaWB result of Lamin B2 Rabbit mAb
Primary antibody: Lamin B2 Rabbit mAb at 1/1000 dilution
Lane 1: mouse spleen lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 68 kDa
Observed MW: 70 kDaWB result of Lamin B2 Rabbit mAb
Primary antibody: Lamin B2 Rabbit mAb at 1/1000 dilution
Lane 1: rat spleen lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 68 kDa
Observed MW: 75 kDa
流式分析
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) labelling Lamin B2 antibody at 1/500 dilution (0.1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫沉淀
Lamin B2 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Lamin B2 in 0.4 mg HeLa whole cell lysate.
Western blot was performed on the immunoprecipitate using Lamin B2 Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: HeLa whole cell lysate 20 µg (Input)
Lane 2: Lamin B2 Rabbit mAb IP in HeLa whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in HeLa whole cell lysate
Predicted MW: 68 kDa
Observed MW: 70 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-Lamin B2 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-Lamin B2 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human testis. Anti-Lamin B2 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human prostatic cancer. Anti-Lamin B2 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human thyroid cancer. Anti-Lamin B2 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-Lamin B2 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse liver. Anti-Lamin B2 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat spleen. Anti-Lamin B2 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat testis. Anti-Lamin B2 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti- Lamin B2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in NIH/3T3 cells. Anti-Lamin B2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
免疫荧光
IF shows positive staining in paraffin-embedded human colon. Anti-Lamin B2 antibody was used at 1/500 dilution (Red) and incubated overnight at 4°C.Goat anti-Rabbit IgG(H+L) (Alexa Fluor® 594 Conjugate) (S0B4007) was used as secondary antibody at 1/1000 dilution.Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded human prostate. Anti-Lamin B2 antibody was used at 1/500 dilution (Red) and incubated overnight at 4°C.Goat anti-Rabbit IgG(H+L) (Alexa Fluor® 594 Conjugate) (S0B4007) was used as secondary antibody at 1/1000 dilution.Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded human testis. Anti-Lamin B2 antibody was used at 1/500 dilution (Red) and incubated overnight at 4°C.Goat anti-Rabbit IgG(H+L) (Alexa Fluor® 594 Conjugate) (S0B4007) was used as secondary antibody at 1/1000 dilution.Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
评论(0)