RBP4 Recombinant Rabbit mAb (S-693-111)

Retinol-binding protein 4,Plasma retinol-binding protein (PRBP,RBP)

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0605

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
分子别名

Retinol-binding protein 4, Plasma retinol-binding protein (PRBP; RBP)
免疫原

Recombinant Protein
细胞定位

Secreted
Accession

P02753
克隆号

S-693-111
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Rt
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

WB

IP
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms, Rt
IP 1:50 Hu
IHC-P 1:500 Hu, Rt

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms, Rt
IP	1:50	Hu
IHC-P	1:500	Hu, Rt

背景介绍

Retinol binding protein 4, also known as RBP4, is a transporter protein[5] for retinol (vitamin A alcohol). RBP4 has a molecular weight of approximately 21 kDa and is encoded by the RBP4 gene in humans. It is mainly, though not exclusively, synthesized in the liver and circulates in the bloodstream as a hepatokine bound to retinol in a complex with transthyretin. RBP4 has been a drug target for ophthalmology research due to its role in vision. RBP4 may also be involved in metabolic diseases as suggested by recent studies.

免疫印迹
- WB result of RPB4 Rabbit mAb
  Primary antibody: RPB4 Rabbit mAb at 1/1000 dilution
  Lane 1: Human serum lysate 5 µg
  Lane 2: Hep G2 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 23 kDa
  Observed MW: 23 kDa
- WB result of RPB4 Rabbit mAb
  Primary antibody: RPB4 Rabbit mAb at 1/1000 dilution
  Lane 1: mouse liver lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 23 kDa
  Observed MW: 23 kDa
- WB result of RPB4 Rabbit mAb
  Primary antibody: RPB4 Rabbit mAb at 1/1000 dilution
  Lane 1: rat liver lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 23 kDa
  Observed MW: 23 kDa
免疫沉淀
- RBP4 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating RBP4 in 0.4 mg human serum lysate.
  Western blot was performed on the immunoprecipitate using RBP4 Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/400 dilution.
  Lane 1: human serum lysate 20 µg (Input)
  Lane 2: RBP4 Rabbit mAb IP in human serum lysate
  Lane 3: Rabbit monoclonal IgG IP in human serum lysate
  Predicted MW: 23 kDa
  Observed MW: 23 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human kidney. Anti-RBP4 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human liver. Anti-RBP4 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-RBP4 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat kidney. Anti-RBP4 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.