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KIM-1 Recombinant Rabbit mAb (S-932-2)

Hepatitis A virus cellular receptor 1,HAVcr-1,Kidney injury molecule 1,T-cell immunoglobulin and mucin domain-containing protein 1 (TIMD-1),T-cell immunoglobulin mucin receptor 1,T-cell membrane protein 1,CD365,HAVCR1,KIM1,TIM1,TIMD1

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0604

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
分子别名

Hepatitis A virus cellular receptor 1, HAVcr-1, Kidney injury molecule 1, T-cell immunoglobulin and mucin domain-containing protein 1 (TIMD-1), T-cell immunoglobulin mucin receptor 1, T-cell membrane protein 1, CD365, HAVCR1, KIM1, TIM1, TIMD1
免疫原

Recombinant Protein
细胞定位

Cell membrane
Accession

Q96D42
克隆号

S-932-2
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20°C as supplied
应用

IHC-P ?

FCM

ICC ?

WB

IP
稀释度

应用稀释度

WB 1:1000
IHC-P 1:500
ICC 1:500
FCM 1:50
IP 1:50

应用	稀释度
WB	1:1000
IHC-P	1:500
ICC	1:500
FCM	1:50
IP	1:50

背景介绍

KIM-1 is a protein the most highly upregulated in injured kidneys by various types of insults. Its upregulation during renal injury has been found in the kidneys of the vertebrates such as Zebrafish and humans. KIM-1 is also a member of the TIM (T cell transmembrane, immunoglobulin, and mucin) gene family, which plays critical roles in regulating immune cell activity especially regarding the host response to viral infection. It is involved in allergic response, asthma, and transplant tolerance.

免疫印迹
- WB result of KIM-1 Rabbit mAb
  Primary antibody: KIM-1 Rabbit mAb at 1/1000 dilution
  Lane 1: HeLa whole cell lysate 20 µg
  Lane 2: A549 whole cell lysate 20 µg
  Negative control: HeLa whole cell lysate
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 39 kDa
  Observed MW: 100~120 kDa
流式分析
- Flow cytometric analysis of HeLa (Human cervix adenocarcinoma epithelial cell, left) / A549 (Human lung carcinoma epithelial cell, right) cells labelling KIM-1 antibody at 1/50 dilution (1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
  Negative control: HeLa
免疫沉淀
- KIM-1 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating KIM-1 in 0.4 mg A549 whole cell lysate.
  Western blot was performed on the immunoprecipitate using KIM-1 Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/1000 dilution.
  Lane 1: A549 whole cell lysate 20 µg (Input)
  Lane 2: KIM-1 Rabbit mAb IP in A549 whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in A549 whole cell lysate
  Predicted MW: 39 kDa
  Observed MW: 100~120 kDa
  This blot was developed with high sensitivity substrate
免疫组化
- IHC shows positive staining in paraffin-embedded human kidney. Anti-KIM-1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-KIM-1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
- ICC shows positive staining in A549 cells. Anti-KIM-1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
- Negative control: ICC shows negative staining in HeLa cells. Anti-KIM-1 antibody was used at 1/500 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).