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宿主来源
Rabbit分子别名
Epididymis tissue protein Li 184a, Profilin-1, PFN1免疫原
Synthetic Peptide细胞定位
Cytoplasm, CytoskeletonAccession
P07737克隆号
S-869-13抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt预测反应种属
(反应种属缩写表)Bv纯化方式
Protein A浓度
0.1 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 WB 1:2000
Profilin1 is a ubiquitous actin monomer-binding protein belonging to the profilin family. It is thought to regulate actin polymerization in response to extracellular signals. Deletion of this gene is associated with Miller-Dieker syndrome. Mutations in this gene may be a rare cause of amyotrophic lateral sclerosis, also called Lou Gehrig's disease.
免疫印迹
WB result of Profilin1 Rabbit mAb
Primary antibody: Profilin1 Rabbit mAb at 1/2000 dilution
Lane 1: HeLa whole cell lysate 5 µg
Lane 2: Jurkat whole cell lysate 5 µg
Lane 3: JAR whole cell lysate 5 µg
Lane 4: Hep G2 whole cell lysate 5 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 15 kDa
Observed MW: 13 kDaWB result of Profilin1 Rabbit mAb
Primary antibody: Profilin1 Rabbit mAb at 1/2000 dilution
Lane 1: NIH/3T3 whole cell lysate 5 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 15 kDa
Observed MW: 13 kDaWB result of Profilin1 Rabbit mAb
Primary antibody: Profilin1 Rabbit mAb at 1/2000 dilution
Lane 1: C6 whole cell lysate 5 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 15 kDa
Observed MW: 13 kDa
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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