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ARID1A Recombinant Rabbit mAb (S-440-27)

AT-rich interactive domain-containing protein 1A,ARID domain-containing protein 1A,B120,BRG1-associated factor 250,BRG1-associated factor 250a,Osa homolog 1,SWI-like protein,SWI/SNF complex protein p270,hELD,BAF250,BAF250A,C1orf4,OSA1,SMARCF1

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0590

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
分子别名

AT-rich interactive domain-containing protein 1A, ARID domain-containing protein 1A, B120, BRG1-associated factor 250, BRG1-associated factor 250a, Osa homolog 1, SWI-like protein, SWI/SNF complex protein p270, hELD, BAF250, BAF250A, C1orf4, OSA1, SMARCF1
免疫原

Recombinant Protein
细胞定位

Nucleus
Accession

O14497
克隆号

S-440-27
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICFCM

IHC-P ?

ICC ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000 Hu
IHC-P 1:500 Hu, Ms, Rt
ICC 1:500 Hu
ICFCM 1:500 Hu

应用	稀释度	推荐种属
WB	1:1000	Hu
IHC-P	1:500	Hu, Ms, Rt
ICC	1:500	Hu
ICFCM	1:500	Hu

背景介绍

ARID1A is part of the large ATP-dependent chromatin remodelling complex SWI/SNF, which is required for transcriptional activation of genes normally repressed by chromatin. It is thought that the protein confers specificity to the SWI/SNF complex and may recruit the complex to its targets through either protein-DNA or protein-protein interactions. ARID1A has been commonly found mutated in gastric cancers, ovarian clear cell carcinoma,[9] and pancreatic cancer. In breast cancer distant metastases acquire inactivation mutations in ARID1A not seen in the primary tumor, and reduced ARID1A expression confers resistance to different drugs such as trastuzumab and mTOR inhibitors.

免疫印迹
- WB result of ARID1A Rabbit mAb
  Primary antibody: ARID1A Rabbit mAb at 1/1000 dilution
  Lane 1: T-47D whole cell lysate 20 µg
  Lane 2: SH-SY5Y whole cell lysate 20 µg
  Negative control: T-47D whole cell lysate
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 242 kDa
  Observed MW: 270 kDa
流式分析
- Flow cytometric analysis of T-47D (Human ductal breast epithelial tumor epithelial cell, Left) / SH-SY5Y (Human neuroblastoma epithelial cell, Right) labelling ARID1A antibody at 1/500 dilution (0.1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
  Negative control: T-47D
免疫组化
- IHC shows positive staining in paraffin-embedded human kidney. Anti-ARID1A antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human renal clear cell carcinoma. Anti-ARID1A antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human breast cancer. Anti-ARID1A antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human endometrial carcinoma. Anti-ARID1A antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human ovarian carcinoma. Anti-ARID1A antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-ARID1A antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-ARID1A antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
- ICC shows positive staining in SH-SY5Y cells (top panel) and negative staining in T-47D cells (below panel). Anti-ARID1A antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).