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PKM Recombinant Rabbit mAb (S-812-20)

Pyruvate kinase PKM,Cytosolic thyroid hormone-binding protein (CTHBP),Opa-interacting protein 3(OIP-3),Pyruvate kinase 2/3,Pyruvate kinase muscle isozyme,Threonine-protein kinase PKM2,Thyroid hormone-binding protein 1 (THBP1),Tumor M2-PK,Tyrosine-protein kinase PKM2,p58,OIP3,PK2,PK3,PKM2

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0588

规格

25μl100μl1ml

数量

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产品规格

宿主来源

Rabbit
分子别名

Pyruvate kinase PKM, Cytosolic thyroid hormone-binding protein (CTHBP), Opa-interacting protein 3(OIP-3), Pyruvate kinase 2/3, Pyruvate kinase muscle isozyme, Threonine-protein kinase PKM2, Thyroid hormone-binding protein 1 (THBP1), Tumor M2-PK, Tyrosine-protein kinase PKM2, p58, OIP3 , PK2, PK3, PKM2
免疫原

Synthetic Peptide
细胞定位

Nucleus, Cytoplasm
Accession

P14618
克隆号

S-812-20
抗体类型

Recombinant mAb
抗体同种型

IgG
应用

ICFCM ,
IHC-P ? ,
ICC ? ,
WB ,
IP
稀释度

应用稀释度

WB 1:1000
IP 1:50
IHC-P 1:500
ICC 1:500
ICFCM 1:500
反应种属 ?

Hu, Ms, Rt
预测反应种属
(反应种属缩写表)

Or, Ct, Rb
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied

应用	稀释度
WB	1:1000
IP	1:50
IHC-P	1:500
ICC	1:500
ICFCM	1:500

背景介绍

Pyruvate kinase is the enzyme involved in the last step of glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to adenosine diphosphate (ADP), yielding one molecule of pyruvate and one molecule of ATP. In contrast to mitochondrial respiration, energy regeneration by pyruvate kinase is independent from oxygen supply and allows survival of the organs under hypoxic conditions often found in solid tumors. Pyruvate kinase is present in four distinct, tissue-specific isozymes in animals, each consisting of particular kinetic properties necessary to accommodate the variations in metabolic requirements of diverse tissues.

免疫印迹
- WB result of PKM Rabbit mAb
  Primary antibody: PKM Rabbit mAb at 1/1000 dilution
  Lane 1: HeLa whole cell lysate 20 µg
  Lane 2: Jurkat whole cell lysate 20 µg
  Lane 3: A549 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 58 kDa
  Observed MW: 60 kDa
  (This blot was developed with high sensitivity substrate)
- WB result of PKM Rabbit mAb
  Primary antibody: PKM Rabbit mAb at 1/1000 dilution
  Lane 1: NIH/3T3 whole cell lysate 20 µg
  Lane 2: mouse lung lysate 20 µg
  Lane 3: mouse skeletal muscle lysate 20 µg
  Lane 4: mouse brain lysate 20 µg
  Lane 5: mouse spleen lysate 20 µg
  Lane 6: mouse testis lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 58 kDa
  Observed MW: 60 kDa
- WB result of PKM Rabbit mAb
  Primary antibody: PKM Rabbit mAb at 1/1000 dilution
  Lane 1: rat skeletal muscle lysate 20 µg
  Lane 2: rat brain lysate 20 µg
  Lane 3: rat testis lysate 20 µg
  Lane 4: rat spleen lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 58 kDa
  Observed MW: 60 kDa
流式分析
- Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling α-tubulin antibody at 1/500 dilution (0.1 μg) / (red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫沉淀
- PKM Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating PKM in 0.4 mg HeLa whole cell lysate.
  Western blot was performed on the immunoprecipitate using PKM Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/400 dilution.
  Lane 1: HeLa whole cell lysate 10 µg (Input)
  Lane 2: PKM Rabbit mAb IP in HeLa whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in HeLa whole cell lysate
  Predicted MW: 58 kDa
  Observed MW: 60 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human kidney. Anti-PKM antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-PKM antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human endometrial carcinoma. Anti-PKM antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-PKM antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-PKM antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
- ICC shows positive staining in HeLa cells. Anti-PKM antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).