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SQSTM1/p62 Recombinant Rabbit mAb (S-778-1)

Sequestosome-1,EBI3-associated protein of 60 kDa (EBIAP,p60),Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa,Ubiquitin-binding protein p62,ORCA,OSIL

货号： S0B0586

Datasheet COA
  • 价格： ￥2,100
  • 规格：
  • 数量：
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产品介绍 引用文献(1) 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 分子别名

    Sequestosome-1, EBI3-associated protein of 60 kDa (EBIAP, p60), Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa, Ubiquitin-binding protein p62, ORCA, OSIL

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Cytoplasm

  • Accession

    Q13501

  • 克隆号

    S-778-1

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 应用

    ICFCM, IHC-P, ICC, WB, IP

  • 反应种属 ?

    Hu, Ms, Rt

  • 预测反应种属
    (反应种属缩写表)

    Or

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied.
稀释度
应用 稀释度
WB 1:1000
IP 1:50
IHC-P 1:500
ICC 1:500
ICFCM 1:500
背景介绍

  • Sequestosome-1 is a protein that in humans is encoded by the SQSTM1 gene. Also known as the ubiquitin-binding protein p62, it is an autophagosome cargo protein that targets other proteins that bind to it for selective autophagy. By interacting with GATA4 and targeting it for degradation, it can inhibit GATA-4 associated senescence and senescence-associated secretory phenotype. Mutations in SQSTM1 are a common cause of Paget's disease of bone.

  • 免疫印迹

    • WB result of SQSTM1/p62 Rabbit mAb
      Primary antibody: SQSTM1/p62 Rabbit mAb at 1/1000 dilution
      Lane 1: HeLa whole cell lysate 20 µg
      Lane 2: Jurkat whole cell lysate 20 µg
      Lane 3: Hep G2 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 48 kDa
      Observed MW: 62 kDa
      (This blot was developed with high sensitivity substrate)

    • WB result of SQSTM1/p62 Rabbit mAb
      Primary antibody: SQSTM1/p62 Rabbit mAb at 1/1000 dilution
      Lane 1: mouse brain lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 48 kDa
      Observed MW: 62 kDa
      (This blot was developed with high sensitivity substrate)

    • WB result of SQSTM1/p62 Rabbit mAb
      Primary antibody: SQSTM1/p62 Rabbit mAb at 1/1000 dilution
      Lane 1: rat brain lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 48 kDa
      Observed MW: 62 kDa
      (This blot was developed with high sensitivity substrate)

  • 流式分析

    • Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HepG2 (Human hepatocellular carcinoma epithelial cell) cells labelling SQSTM1/p62 antibody at 1/500 dilution (0.1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.

  • 免疫沉淀

    • SQSTM1/p62 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating SQSTM1/p62 in 0.4 mg HeLa whole cell lysate.
      Western blot was performed on the immunoprecipitate using SQSTM1/p62 Rabbit mAb at 1/1000 dilution.
      Secondary antibody (HRP) for IP was used at 1/400 dilution.
      Lane 1: HeLa whole cell lysate 20 µg (Input)
      Lane 2: SQSTM1/p62 Rabbit mAb IP in HeLa whole cell lysate
      Lane 3: Rabbit monoclonal IgG IP in HeLa whole cell lysate
      Predicted MW: 48 kDa
      Observed MW: 62 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human colon. Anti-SQSTM1/p62 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human colon cancer. Anti-SQSTM1/p62 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-SQSTM1/p62 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human testis. Anti-SQSTM1/p62 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-SQSTM1/p62 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat liver. Anti-SQSTM1/p62 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫细胞化学

    • ICC analysis of HeLa cells treated with Chloroquine (50μM, 24 hours) (top panel) and HeLa cells untreated with Chloroquine (50μM, 24 hours) (below panel). Anti-SQSTM1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

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