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CEBP Beta (C-terminal) Recombinant Rabbit mAb (S-773-48)

CCAAT/enhancer-binding protein beta,C/EBP beta,Liver activator protein (LAP),Liver-enriched inhibitory protein (LIP),Nuclear factor NF-IL6,Transcription factor 5 (TCF-5),CEBPB,TCF5

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0582

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
分子别名

CCAAT/enhancer-binding protein beta; C/EBP beta; Liver activator protein (LAP); Liver-enriched inhibitory protein (LIP); Nuclear factor NF-IL6; Transcription factor 5 (TCF-5); CEBPB; TCF5
免疫原

Synthetic Peptide
细胞定位

Cytoplasm, Nucleus
Accession

P17676
克隆号

S-773-48
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20°C as supplied
应用

ICFCM

ChIP

ICC ?

WB
稀释度

应用稀释度

WB 1:1000
ICC 1:500
ICFCM 1:500
ChIP 1:20-1:50

应用	稀释度
WB	1:1000
ICC	1:500
ICFCM	1:500
ChIP	1:20-1:50

背景介绍

CEBP beta is a bZIP transcription factor that can bind as a homodimer to certain DNA regulatory regions. It can also form heterodimers with the related proteins CEBP-alpha, CEBP-delta, and CEBP-gamma. It is important in the regulation of genes involved in immune and inflammatory responses and has been shown to bind to the IL-1 response element in the IL-6 gene, as well as to regulatory regions of several acute-phase and cytokine genes. In addition, CEBP beta can bind the promoter and upstream element and stimulate the expression of the collagen type I gene.

免疫印迹
- WB result of CEBP Beta (C-terminal) Rabbit mAb
  Primary antibody: CEBP Beta (C-terminal) Rabbit mAb at 1/1000 dilution
  Lane 1: HeLa whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 38 kDa
  Observed MW: 20, 40, 45 kDa
  Exposure time: 120 s (This blot was developed with high sensitivity substrate)
- WB result of CEBP Beta (C-terminal) Rabbit mAb
  Primary antibody: CEBP Beta (C-terminal) Rabbit mAb at 1/1000 dilution
  Lane 1: NIH/3T3 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 38 kDa
  Observed MW: 20, 35, 38 kDa
  Exposure time: 120 s (This blot was developed with high sensitivity substrate)
- WB result of CEBP Beta (C-terminal) Rabbit mAb
  Primary antibody: CEBP Beta (C-terminal) Rabbit mAb at 1/1000 dilution
  Lane 1: PC-12 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 38 kDa
  Observed MW: 20, 35, 38 kDa
  Exposure time: 120 s (This blot was developed with high sensitivity substrate)
流式分析
- Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelled with anti-CEBP Beta (C-terminal) antibody at 1/500 dilution (0.1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
- Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast) cells labelled with anti-CEBP Beta (C-terminal) antibody at 1/500 dilution (0.1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫细胞化学
- ICC shows positive staining in HeLa cells. Anti- CEBP Beta (C-terminal) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
- ICC shows positive staining in NIH/3T3 cells. Anti-CEBP Beta (C-terminal) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ChIP
- Chromatin immunoprecipitation (ChIP) was performed on HeLa cells cross-linked with 1% formaldehyde for 10 min, then chromatin was fragmented by sonication. Parallel reactions used CEBP Beta (C-terminal) Recombinant Rabbit mAb (S-773-48) and Rabbit mAb IgG Isotype Control (SDT-R173) at 1:50 for immunoprecipitation.
  Post - immunoprecipitation, both samples were washed, eluted, and cross-links reversed. Purified DNA was analyzed by qPCR.
  qPCR showed the enrichment of IL-6, REL and SAT-2 in CEBP Beta (C-terminal)
  Recombinant Rabbit mAb (S-773-48)-immunoprecipitated sample.