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Invivo anti-mouse CTLA-4 (CD152) mAb

Cytotoxic T-lymphocyte protein 4,Cytotoxic T-lymphocyte-associated antigen 4

货号： S0B0574

Datasheet COA
  • 价格： ￥700
  • 规格：
  • 数量：
斯达特大包装询价 大包装询价

产品介绍 评论(0)

产品规格

  • 宿主来源

    Mouse

  • 分子别名

    Cytotoxic T-lymphocyte protein 4, Cytotoxic T-lymphocyte-associated antigen 4

  • 细胞定位

    Cell membrane

  • Accession

    P09793

  • 克隆号

    9D9

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG2b,k

  • 应用

    in vivo CTLA-4 neutralizatior, WB

  • 反应种属 ?

    Ms

  • 纯化方式

    Protein A

  • 浓度

    Lot specific* (generally 5 to 20 mg/ml)*

  • 内毒素含量

    <1EU/mg

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS pH7.4, containing no preservative

  • 储存条件

    2 to 8 °C for 2 weeks under sterile conditions;

    -20 °C for 3 months under sterile conditions;

    -80 °C for 24 months under sterile conditions.

    Please avoid repeated freeze-thaw cycles.

稀释度
应用 稀释度
WB 1:1000
背景介绍

  • This antibody recognizes the same epitope as clone 9D9.

    Cytotoxic T-lymphocyte associated protein 4, (CTLA-4) also known as CD152 (cluster of differentiation 152), is a protein receptor that functions as an immune checkpoint and downregulates immune responses. CTLA-4 is constitutively expressed in regulatory T cells but only upregulated in conventional T cells after activation – a phenomenon which is particularly notable in cancers. CTLA-4 may also function via modulation of cell motility and/or signaling through PI3 kinase.

  • 验证数据

    • Animal species and strain: BALB/c

      Cell line: EMT6

    • Animal species and strain: BALB/c

      Cell line: EMT6

  • 免疫印迹

    • WB result of Invivo CTLA-4 (CD152) Mouse mAb
      Primary antibody: Invivo CTLA-4 (CD152) Mouse mAb at 1/1000 dilution
      Lane 1: reduced purified mouse CTLA-4 with histidine tag at C-terminus 1 µg
      Lane 2: reduced purified mouse CTLA-4 with histidine tag at C-terminus 0.5 µg
      Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 25~30 kDa
      Observed MW: 20~30 kDa

  • SPR

    • Anti-His antibody Immobilized on CM5 Chip captured CD152 His Tag, Mouse, can bind CTLA-4 (CD152) Mouse mAb (9D9) with an affinity constant of 2.315 nM as determined in SPR assay.

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