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宿主来源
Mouse抗原名称
CD90分子别名
Thy-1 membrane glycoprotein, CDw90, Thy-1 antigen, THY1免疫原
Recombinant Protein细胞定位
Cell membraneAccession
P04216克隆号
S-922-13抗体类型
Mouse mAb抗体同种型
IgG1,k应用
FCM反应种属 ?
Hu纯化方式
Protein G浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用 | 稀释度 |
---|---|
FCM | 1:2000 |
Thy-1 or CD90 (Cluster of Differentiation 90) is a ~25–37 kDa heavily N-glycosylated, glycophosphatidylinositol (GPI) anchored conserved cell surface protein with a single V-like immunoglobulin domain, originally discovered as a thymocyte antigen. Thy-1 can be used as a marker for a variety of stem cells and for the axonal processes of mature neurons. Structural study of Thy-1 led to the foundation of the Immunoglobulin superfamily, of which it is the smallest member, and led to some of the initial biochemical description and characterization of a vertebrate GPI anchor and also the first demonstration of tissue specific differential glycosylation.
流式分析
Flow cytometric analysis of K562 (Human chronic myelogenous leukemia lymphoblast, Left) / U-2 OS (Human bone osteosarcoma epithelial cell, Right) labelling CD90 antibody at 1/2000 dilution (0.1 μg) / (Red) compared with a Mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Mouse IgG Alexa Fluor® 488 was used as the secondary antibody.
Negative control: K562
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