CD31 Mouse mAb (S-934-6)

PECAM-1,EndoCAM,GPIIA,PECA1

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0551

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Mouse
抗原名称

CD31
分子别名

PECAM-1, EndoCAM, GPIIA, PECA1
免疫原

Recombinant Protein
细胞定位

Cell membrane
Accession

P16284
克隆号

S-934-6
抗体类型

Mouse mAb
抗体同种型

IgG1,k
反应种属 ?

Hu
纯化方式

Protein G
浓度

2 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

FCM

ICC ?

WB
稀释度

应用稀释度

WB 1:1000
ICC 1:100
FCM 1:200

应用	稀释度
WB	1:1000
ICC	1:100
FCM	1:200

背景介绍

Cluster of differentiation 31 (CD31) also known as platelet endothelial cell adhesion molecule (PECAM-1) is a member of the immunoglobulin superfamily and is likely involved in leukocyte transmigration, angiogenesis, and integrin activation. PECAM-1 plays a key role in removing aged neutrophils from the body. PECAM-1 is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. In immunohistochemistry, CD31 is used primarily to demonstrate the presence of endothelial cells in histological tissue sections. This can help to evaluate the degree of tumor angiogenesis, which can imply a rapidly growing tumor. Malignant endothelial cells also commonly retain the antigen, so that CD31 immunohistochemistry can also be used to demonstrate both angiomas and angiosarcomas. It can also be demonstrated in small lymphocytic and lymphoblastic lymphomas, although more specific markers are available for these conditions.

免疫印迹
- WB result of CD31 Mouse mAb
  Primary antibody: CD31 Mouse mAb at 1/1000 dilution
  Lane 1: HeLa whole cell lysate 20 µg
  Lane 2: THP-1 whole cell lysate 20 µg
  Negative control: HeLa whole cell lysate
  Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 82 kDa
  Observed MW: 130 kDa
  (This blot was developed with high sensitivity substrate)
流式分析
- Flow cytometric analysis of HeLa (Human cervix adenocarcinoma epithelial cell, left) / Jurkat (Human T cell leukemia T lymphocyte, right) cells labelling CD31 antibody at 1/200 dilution (1 μg)/ (Red) compared with a Mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Mouse IgG Alexa Fluor® 488 was used as the secondary antibody.
  Negative control: HeLa
免疫细胞化学
- ICC shows positive staining in Jurkat cells. Anti-CD31 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
- Negative control: ICC shows negative staining in HeLa cells. Anti-CD31 antibody was used at 1/100 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).