申请试用 
分享产品介绍 FAQs 评论(0)
宿主来源
Rabbit分子别名
H3K4/9/18ac免疫原
Synthetic Peptide细胞定位
NucleusAccession
P68431抗体类型
Polyclonal antibody抗体同种型
IgG翻译后修饰类型
乙酰化应用
ChIP ,IHC-P ? ,WB ,IF ?反应种属 ?
Hu, Ms, Rt预测反应种属
(反应种属缩写表)Fu, Pl, Ys, SeUr, Dr, C.el, Hm, Or, Bv, Xe纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 |
|---|---|
| Dot Blot | 1:1000 |
| WB | 1:1000 |
| IHC-P | 1:500 |
| IF | 1:500 |
| ChIP | 1:20~1:50 |
Acetylated histones, octameric proteins that organize chromatin into nucleosomes, the basic structural unit of the chromosomes and ultimately higher order structures, represent a type of epigenetic marker within chromatin. Acetylation removes the positive charge on the histones, thereby decreasing the interaction of the N termini of histones with the negatively charged phosphate groups of DNA. As a consequence, the condensed chromatin is transformed into a more relaxed structure that is associated with greater levels of gene transcription.
免疫印迹
WB result of Histone H3 (acetyl K4 + K9 + K18) Rabbit pAb
Primary antibody: Histone H3 (acetyl K4 + K9 + K18) Rabbit pAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with Trichostatin A (100 ng/ml, 4 hour ) whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 15 kDa
Observed MW: 17 kDa
Exposure time: 24 sWB result of Histone H3 (acetyl K4 + K9 + K18) Rabbit pAb
Primary antibody: Histone H3 (acetyl K4 + K9 + K18) Rabbit pAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: NIH/3T3 treated with Trichostatin A (100 ng/ml, 4 hour ) whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 15 kDa
Observed MW: 17 kDa
Exposure time: 24 sWB result of Histone H3 (acetyl K4 + K9 + K18) Rabbit pAb
Primary antibody: Histone H3 (acetyl K4 + K9 + K18) Rabbit pAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: C6 treated with Trichostatin A (100 ng/ml, 4 hour ) whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 15 kDa
Observed MW: 17 kDa
Exposure time: 24 s
斑点杂交
Dot blot result of Histone H3 (acetyl K4 + K9 + K18) Rabbit pAb
Lane 1: H3 (acetyl K18) peptide
Lane 2: H3 (acetyl K4) peptide
Lane 3: H3 (acetyl K9) peptide
Lane 4: H3 unmodified peptide
Primary antibody: Histone H3 (acetyl K4 + K9 + K18) Rabbit pAb at 1/1000 dilution
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Exposure time: 40 s
免疫组化
IHC shows positive staining in paraffin-embedded human testis. Anti-Histone H3 (acetyl K4 + K9 + K18) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti- Histone H3 (acetyl K4 + K9 + K18) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti- Histone H3 (acetyl K4 + K9 + K18) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human thyroid cancer. Anti- Histone H3 (acetyl K4 + K9 + K18) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse stomach. Anti- Histone H3 (acetyl K4 + K9 + K18) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat liver. Anti- Histone H3 (acetyl K4 + K9 + K18) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
IF shows positive staining in paraffin-embedded human stomach. Anti-Histone H3 (acetyl K4 + K9 + K18) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
ChIP
Chromatin immunoprecipitation (ChIP) was performed on HeLa cells cross - linked with 1% formaldehyde for 10 min, then chromatin was fragmented by sonication. Parallel reactions used Histone H3 (acetyl K4 + K9 + K18) Rabbit polyclonal antibody and Rabbit mAb IgG Isotype Control (SDT-R173) at 1:50 for immunoprecipitation. Post - immunoprecipitation, both samples were washed, eluted, and cross - links reversed. Purified DNA was analyzed by qPCR.
qPCR (%input: immunoprecipitated DNA/input DNA) showed the enrichment of RPL30, GAPDH, MYOD1, AFM, SAT-α and SAT-2 in Histone H3 (acetyl K4 + K9 + K18) Rabbit polyclonal antibody - immunoprecipitated sample.
-
斯达特公司的抗体，可以回收利用几次？
评论(0)