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Rabbit分子别名
H3K79me2免疫原
Synthetic Peptide细胞定位
NucleusAccession
P68431抗体类型
Polyclonal antibody抗体同种型
IgG应用
ICFCM, ChIP, ICC, WB反应种属 ?
Hu, Ms预测反应种属
(反应种属缩写表)Fu, Pl, Ys, SeUr, Dr, C.el, Hm, Or, Bv, Xe纯化方式
Protein A浓度
0.8 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 |
|---|---|
| Dot Blot | 1:1000 |
| WB | 1:1000 |
| ICC | 1:500 |
| ICFCM | 1:800 |
| ChIP | 1:20-1:50 |
H3K79me2 is an epigenetic modification to the DNA packaging protein Histone H3. It is a mark that indicates the di-methylation at the 79th lysine residue of the histone H3 protein. H3K79me2 is detected in the transcribed regions of active genes.
免疫印迹
WB result of Histone H3 (di methyl K79) Rabbit pAb
Primary antibody: Histone H3 (di methyl K79) Rabbit pAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 17 kDa
Observed MW: 17 kDa
Exposure time: 3 s (This blot was developed with high sensitivity substrate)WB result of Histone H3 (di methyl K79) Rabbit pAb
Primary antibody: Histone H3 (di methyl K79) Rabbit pAb at 1/1000 dilution
Lane 1: Neuro-2a whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 17 kDa
Observed MW: 17 kDa
Exposure time: 3 s (This blot was developed with high sensitivity substrate)
流式分析
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) labelling Histone H3 (di methyl K79) antibody at 1/800 dilution (0.1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti-Histone H3 (di methyl K79) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
配对推荐
Dot blot result of Histone H3 (di methyl K79) Rabbit pAb
Lane 1: H3 (di methyl K79) peptide
Lane 2: H3 (mono methyl K79) peptide
Lane 3: H3 (tri methyl K79) peptide
Lane 4: H3 unmodified peptide
Primary antibody: Histone H3 (di methyl K79) Rabbit pAb at 1/1000 dilution
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Exposure time: 90 s
ChIP
Chromatin immunoprecipitation (ChIP) was
performed on HeLa cells cross - linked with
1% formaldehyde for 10 min, then chromatin
was fragmented by sonication.
Parallel reactions used Histone H3 (di methyl
K79) Recombinant Rabbit mAb (S-861-22) and
IgG Isotype Control (SDT-R173) at 1:50 for
immunoprecipitation.
Post - immunoprecipitation, both samples
were washed, eluted, and cross - links reversed.
Purified DNA was analyzed by qPCR.
qPCR showed the enrichment of RPL30, GAPDH,
MYOD1, AFM, SAT-α and SAT-2 in Histone H3
(di methyl K79) Recombinant Rabbit mAb (S-861-22)-
immunoprecipitated sample.
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