Histone H3 (di methyl K79) Rabbit polyclonal antibody

H3K79me2

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0544

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
分子别名

H3K79me2
免疫原

Synthetic Peptide
细胞定位

Nucleus
Accession

P68431
抗体类型

Polyclonal antibody
抗体同种型

IgG
翻译后修饰类型

甲基化
反应种属 ?

Hu, Ms
预测反应种属
(反应种属缩写表)

Fu, Pl, Ys, SeUr, Dr, C.el, Hm, Or, Bv, Xe
纯化方式

Protein A
浓度

0.8 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICFCM

ChIP

ICC ?

WB
稀释度

应用稀释度推荐种属

Dot Blot 1:1000
WB 1:1000 Hu, Ms
ICC 1:500 Hu
ICFCM 1:800 Hu
ChIP 1:20-1:50 Hu

应用	稀释度	推荐种属
Dot Blot	1:1000
WB	1:1000	Hu, Ms
ICC	1:500	Hu
ICFCM	1:800	Hu
ChIP	1:20-1:50	Hu

背景介绍

H3K79me2 is an epigenetic modification to the DNA packaging protein Histone H3. It is a mark that indicates the di-methylation at the 79th lysine residue of the histone H3 protein. H3K79me2 is detected in the transcribed regions of active genes.

免疫印迹
- WB result of Histone H3 (di methyl K79) Rabbit pAb
  Primary antibody: Histone H3 (di methyl K79) Rabbit pAb at 1/1000 dilution
  Lane 1: HeLa whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 17 kDa
  Observed MW: 17 kDa
  Exposure time: 3 s (This blot was developed with high sensitivity substrate)
- WB result of Histone H3 (di methyl K79) Rabbit pAb
  Primary antibody: Histone H3 (di methyl K79) Rabbit pAb at 1/1000 dilution
  Lane 1: Neuro-2a whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 17 kDa
  Observed MW: 17 kDa
  Exposure time: 3 s (This blot was developed with high sensitivity substrate)
流式分析
- Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) labelling Histone H3 (di methyl K79) antibody at 1/800 dilution (0.1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫细胞化学
- ICC shows positive staining in HeLa cells. Anti-Histone H3 (di methyl K79) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
配对推荐
- Dot blot result of Histone H3 (di methyl K79) Rabbit pAb
  Lane 1: H3 (di methyl K79) peptide
  Lane 2: H3 (mono methyl K79) peptide
  Lane 3: H3 (tri methyl K79) peptide
  Lane 4: H3 unmodified peptide
  Primary antibody: Histone H3 (di methyl K79) Rabbit pAb at 1/1000 dilution
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Exposure time: 90 s
ChIP
- Chromatin immunoprecipitation (ChIP) was
  performed on HeLa cells cross - linked with
  1% formaldehyde for 10 min, then chromatin
  was fragmented by sonication.
  Parallel reactions used Histone H3 (di methyl
  K79) Recombinant Rabbit mAb (S-861-22) and
  IgG Isotype Control (SDT-R173) at 1:50 for
  immunoprecipitation.
  Post - immunoprecipitation, both samples
  were washed, eluted, and cross - links reversed.
  Purified DNA was analyzed by qPCR.
  qPCR showed the enrichment of RPL30, GAPDH,
  MYOD1, AFM, SAT-α and SAT-2 in Histone H3
  (di methyl K79) Recombinant Rabbit mAb (S-861-22)-
  immunoprecipitated sample.