PTP1B Recombinant Rabbit mAb (S-R304)

Tyrosine-protein phosphatase non-receptor type 1,Protein-tyrosine phosphatase 1B (PTP-1B),PTPN1

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0543

规格

25μl100μl1ml

数量

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产品规格

宿主来源

Rabbit
抗原名称

PTP1B
分子别名

Tyrosine-protein phosphatase non-receptor type 1, Protein-tyrosine phosphatase 1B (PTP-1B), PTPN1
细胞定位

Endoplasmic reticulum, Membrane
Accession

P18031
克隆号

S-R304
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20°C as supplied
应用

IHC-P ? ,
WB ,
IP
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms
IP 1:50 Hu
IHC-P 1:2000 Hu

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms
IP	1:50	Hu
IHC-P	1:2000	Hu

背景介绍

PTP1B was first isolated from a human placental protein extract, but it is expressed in many tissues. It is localized to the cytoplasmic face of the endoplasmic reticulum. PTP1B can dephosphorylate the phosphotyrosine residues of the activated insulin receptor kinase. PTP1B is a negative regulator of the insulin signaling pathway and is considered a promising potential therapeutic target, in particular for treatment of type 2 diabetes. It has also been implicated in the development of breast cancer and has been explored as a potential therapeutic target in that avenue as well.

免疫印迹
- WB result of PTP1B Rabbit mAb
  Primary antibody: PTP1B Rabbit mAb at 1/1000 dilution
  Lane 1: HeLa whole cell lysate 20 µg
  Lane 2: HCT 116 whole cell lysate 20 µg
  Lane 3: HepG2 whole cell lysate 20 µg
  Lane 4: A549 whole cell lysate 20 µg
  Lane 5: MCF7 whole cell lysate 20 µg
  Lane 6: SW480 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 50 kDa
  Observed MW: 50 kDa
- WB result of PTP1B Rabbit mAb
  Primary antibody: PTP1B Rabbit mAb at 1/1000 dilution
  Lane 1: RAW 264.7 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 50 kDa
  Observed MW: 50 kDa
  (This blot was developed with high sensitivity substrate)
免疫沉淀
- PTP1B Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating PTP1B in 0.4 mg HCT116 whole cell lysate.
  Western blot was performed on the immunoprecipitate using PTP1B Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/400 dilution.
  Lane 1: HCT116 whole cell lysate 10 µg (Input)
  Lane 2: PTP1B Rabbit mAb IP in HCT116 whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in HCT116 whole cell lysate
  Predicted MW: 50 kDa
  Observed MW: 50 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human bladder cancer. Anti-PTP1B antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human lung cancer. Anti-PTP1B antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-PTP1B antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human pancreatic cancer. Anti-PTP1B antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.