PDGFRA+PDGFRB Recombinant Rabbit mAb,PBS Only (S-393-11)

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货号 S0B0541P

规格

100μg1mg

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

PDGFRA+PDGFRB
免疫原

Synthetic Peptide
细胞定位

Cell membrane
Accession

P09619, P16234
克隆号

S-393-11
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu
纯化方式

Protein A
浓度

1 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS pH7.4
储存条件

12 months from date of receipt / reconstitution, 4 °C as supplied
应用

IHC-P ?

WB
稀释度

应用稀释度

WB 1:1000
IHC-P 1:1000

应用	稀释度
WB	1:1000
IHC-P	1:1000

背景介绍

Platelet-derived growth factor receptors (PDGF-R) are cell surface tyrosine kinase receptors for members of the platelet-derived growth factor (PDGF) family. PDGF subunits -A and -B are important factors regulating cell proliferation, cellular differentiation, cell growth, development and many diseases including cancer. There are two forms of the PDGF-R, alpha and beta each encoded by a different gene. Depending on which growth factor is bound, PDGF-R homo- or heterodimerizes.

免疫印迹
- WB result of PDGFRA+PDGFRB Rabbit mAb
  Primary antibody: PDGFRA+PDGFRB Rabbit mAb at 1/1000 dilution
  Lane 1: SH-SY5Y whole cell lysate 40 µg
  Lane 2: U-87 MG whole cell lysate 40 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 124 kDa
  Observed MW: 200 kDa
  Exposure time: 60 s
免疫组化
- IHC shows positive staining in paraffin-embedded human kidney. Anti-PDGFRA+PDGFRB antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human placenta. Anti-PDGFRA+PDGFRB antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human breast cancer. Anti-PDGFRA+PDGFRB antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-PDGFRA+PDGFRB antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-PDGFRA+PDGFRB antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human pancreatic carcinoma. Anti-PDGFRA+PDGFRB antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.