PDGFRA+PDGFRB Recombinant Rabbit mAb (S-393-11)

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0541

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
免疫原

Synthetic Peptide
细胞定位

Cell membrane
Accession

P09619, P16234
克隆号

S-393-11
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

WB

IP

IF ?
稀释度

应用稀释度

WB 1:1000
IHC-P 1:1000
IF 1:100
IP 1:50

应用	稀释度
WB	1:1000
IHC-P	1:1000
IF	1:100
IP	1:50

背景介绍

Platelet-derived growth factor receptors (PDGF-R) are cell surface tyrosine kinase receptors for members of the platelet-derived growth factor (PDGF) family. PDGF subunits -A and -B are important factors regulating cell proliferation, cellular differentiation, cell growth, development and many diseases including cancer. There are two forms of the PDGF-R, alpha and beta each encoded by a different gene. Depending on which growth factor is bound, PDGF-R homo- or heterodimerizes.

免疫印迹
- WB result of PDGFRA+PDGFRB Rabbit mAb
  Primary antibody: PDGFRA+PDGFRB Rabbit mAb at 1/1000 dilution
  Lane 1: SH-SY5Y whole cell lysate 20 µg
  Lane 2: U-87 MG whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 124 kDa
  Observed MW: 200 kDa
  (This blot was developed with high sensitivity substrate)
免疫沉淀
- PDGFRA+PDGFRB Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating PDGFRA+PDGFRB in 0.4 mg SH-SY5Y whole cell lysate.
  Western blot was performed on the immunoprecipitate using PDGFRA+PDGFRB Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/1000 dilution.
  Lane 1: SH-SY5Y whole cell lysate 20 µg (Input)
  Lane 2: PDGFRA+PDGFRB Rabbit mAb IP in SH-SY5Y whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in SH-SY5Y whole cell lysate
  Predicted MW: 124 kDa
  Observed MW: 200 kDa
  Exposure time: 20 s
免疫组化
- IHC shows positive staining in paraffin-embedded human kidney. Anti-PDGFRA+PDGFRB antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human placenta. Anti-PDGFRA+PDGFRB antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human breast cancer. Anti-PDGFRA+PDGFRB antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-PDGFRA+PDGFRB antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-PDGFRA+PDGFRB antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human pancreatic carcinoma. Anti-PDGFRA+PDGFRB antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
- IF shows positive staining in paraffin-embedded human colon cancer. Anti-PDGFRA+PDGFRB antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
- IF shows positive staining in paraffin-embedded human squamous cell lung carcinoma. Anti-PDGFRA+PDGFRB antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
- IF shows positive staining in paraffin-embedded human endometrial cancer. Anti-PDGFRA+PDGFRB antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.