分享产品介绍 FAQs 评论(0)
宿主来源
Rabbit分子别名
Protein lin-28 homolog A, Lin-28A, Zinc finger CCHC domain-containing protein 1, LIN28A, CSDD1, LIN28, ZCCHC1免疫原
Synthetic Peptide细胞定位
CytoplasmAccession
Q9H9Z2克隆号
S-795-8抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
IP
稀释度
应用 稀释度 WB 1:1000 IP 1:50
an RNA-binding protein that binds to and enhances the translation of the IGF-2 (insulin-like growth factor 2) mRNA. Lin28 binds to the let-7 pre-microRNA and blocks production of the mature let-7 microRNA in mouse embryonic stem cells. In pluripotent embryonal carcinoma cells, LIN28 is localized in the ribosomes, P-bodies and stress granules. LIN28 is highly expressed in human embryonic stem cells[14] and can enhance the efficiency of the formation of induced pluripotent stem (iPS) cells from human fibroblasts. LIN28 is a marker of undifferentiated human embryonic stem cells and has been used to enhance the efficiency of the formation of iPS cells from human fibroblasts.
免疫印迹
WB result of LIN28 Rabbit mAb
Primary antibody: LIN28 Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: JAR whole cell lysate 20 µg
Lane 3: T-47D whole cell lysate 20 µg
Lane 4: NCCIT whole cell lysate 20 µg
Negative control: HeLa whole cell lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 23 kDa
Observed MW: 26 kDa
免疫沉淀
LIN28 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating LIN28 in 0.4 mg T-47D whole cell lysate.
Western blot was performed on the immunoprecipitate using LIN28 Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/400 dilution.
Lane 1: T-47D whole cell lysate 20 µg (Input)
Lane 2: LIN28 Rabbit mAb IP in T-47D whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in T-47D whole cell lysate
Predicted MW: 23 kDa
Observed MW: 26 kDa
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
评论(0)