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宿主来源
Rabbit分子别名
Insulin-like growth factor-binding protein 2, IBP-2, IGF-binding protein 2, BP2, IBP2免疫原
Synthetic Peptide细胞定位
SecretedAccession
P18065克隆号
S-808-24抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 WB 1:1000 IHC-P 1:200
IGFBP2 is one of six homologous proteins in the IGFBP family. Based on its ability to bind IGFs with high affinity, IGFBP2 can exert its functions by interacting with IGFs in the circulation to control the function and activity of IGFs, and prevent the interaction between IGFs and IGF receptors. Moreover, it can also bind to IGFs and confer an IGF-dependent growth inhibitory or stimulatory effect in many cell types. IGFBP2 also performs IGF-independent roles in cancer development and progression through a variety of molecular networks. Aberrant overexpression of IGFBP2 is associated with an aggressive phenotype of a broad range of human cancers, including glioma, ovarian, prostate, pancreatic, breast, lung, colorectal, melanoma, liver cancer, gastric, rhabdomyosarcoma and leukemia. Moreover, high circulating IGFBP2 levels may serve as a usable diagnostic or prognostic tumor biomarker in many types of cancer, and is closely associated with relapse and a poorer outlook for patients with cancer
免疫印迹
WB result of IGFBP2 Rabbit mAb
Primary antibody: IGFBP2 Rabbit mAb at 1/1000 dilution
Lane 1: SK-OV-3 whole cell lysate 20 µg
Lane 2: T-47D whole cell lysate 20 µg
Lane 3: SH-SY5Y whole cell lysate 20 µg
Negative control: SK-OV-3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 35 kDa
Observed MW: 33 kDa
(This blot was developed with high sensitivity substrate)
免疫组化
IHC shows positive staining in paraffin-embedded human colon. Anti-IGFBP2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-IGFBP2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-IGFBP2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human pancreatic cancer. Anti-IGFBP2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-IGFBP2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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