TMEM119 Recombinant Rabbit mAb (S-838-46)

Transmembrane protein 119,Osteoblast induction factor (OBIF)

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0536

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
分子别名

Transmembrane protein 119, Osteoblast induction factor (OBIF)
免疫原

Recombinant Protein
细胞定位

Cytoplasm, Cell membrane
Accession

Q8R138
克隆号

S-838-46
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICFCM

ICC ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms, Rt
ICC 1:500 Hu
ICFCM 1:500 Hu

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms, Rt
ICC	1:500	Hu
ICFCM	1:500	Hu

背景介绍

TMEM119 is a type I transmembrane protein originally identified as a regulator of osteoblast differentiation, which is expressed in several tissues. In the brain, TMEM119 is specific to microglia, and although its role is still unknown, could represent a useful tool to differentiate resident microglia from macrophages. TMEM119 plays an important role in bone formation and normal bone mineralization.

免疫印迹
- WB result of TMEM119 Rabbit mAb
  Primary antibody: TMEM119 Rabbit mAb at 1/1000 dilution
  Lane 1: SH-SY5Y whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 29 kDa
  Observed MW: 55 kDa
  (This blot was developed with high sensitivity substrate)
- WB result of TMEM119 Rabbit mAb
  Primary antibody: TMEM119 Rabbit mAb at 1/1000 dilution
  Lane 1: mouse brain lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 29 kDa
  Observed MW: 55 kDa
  (This blot was developed with high sensitivity substrate)
- WB result of TMEM119 Rabbit mAb
  Primary antibody: TMEM119 Rabbit mAb at 1/1000 dilution
  Lane 1: rat brain lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 29 kDa
  Observed MW: 55 kDa
  (This blot was developed with high sensitivity substrate)
流式分析
- Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized SH-SY5Y (Human neuroblastoma epithelial cell) cells labelling TMEM119 antibody at 1/500 dilution (0.1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫细胞化学
- ICC shows positive staining in SH-SY5Y cells. Anti-TMEM119 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).