Myosin light chain 3 Recombinant Rabbit mAb (S-664-75)
Cardiac myosin light chain 1,CMLC1,Myosin light chain 1,slow-twitch muscle B/ventricular isoform,MLC1SB,Ventricular myosin alkali light chain,Ventricular myosin light chain 1,VLCl,Ventricular/slow twitch myosin alkali light chain,MLC-lV/sb,MYL3
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宿主来源
Rabbit抗原名称
Myosin light chain 3分子别名
Cardiac myosin light chain 1, CMLC1, Myosin light chain 1, slow-twitch muscle B/ventricular isoform, MLC1SB, Ventricular myosin alkali light chain, Ventricular myosin light chain 1, VLCl, Ventricular/slow twitch myosin alkali light chain, MLC-lV/sb, MYL3免疫原
Synthetic Peptide细胞定位
CytosolAccession
P08590克隆号
S-664-75抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Ms, Rt IHC-P 1:2000 Hu, Ms, Rt
Myosin essential light chain (ELC), ventricular/cardiac isoform is a protein that in humans is encoded by the MYL3 gene. This cardiac ventricular/slow skeletal ELC isoform is distinct from that expressed in fast skeletal muscle (MYL1) and cardiac atrial muscle (MYL4). Ventricular ELC is part of the myosin molecule and is important in modulating cardiac muscle contraction. Mutations in MYL3 have been identified as a cause of familial hypertrophic cardiomyopathy, and associated with a mid-left ventricular chamber type hypertrophy.
免疫印迹
WB result of Myosin light chain 3 Rabbit mAb
Primary antibody: Myosin light chain 3 Rabbit mAb at 1/1000 dilution
Lane 1: mouse heart lysate 20 µg
Lane 2: mouse skeletal muscle lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 22 kDa
Observed MW: 24 kDaWB result of Myosin light chain 3 Rabbit mAb
Primary antibody: Myosin light chain 3 Rabbit mAb at 1/1000 dilution
Lane 1: rat heart lysate 5 µg
Lane 2: rat skeletal muscle lysate 5 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 22 kDa
Observed MW: 22 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-Myosin light chain 3 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human skeletal muscle. Anti-Myosin light chain 3 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cardiac muscle. Anti-Myosin light chain 3 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cardiac muscle. Anti-Myosin light chain 3 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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