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宿主来源
Rabbit分子别名
Tumor necrosis factor receptor superfamily member 14, Herpes virus entry mediator A, HveA, Tumor necrosis factor receptor-like 2, TR2, CD270, HVEA, HVEM免疫原
Recombinant Protein细胞定位
Cell membraneAccession
Q92956克隆号
S-727-70抗体类型
Recombinant mAb抗体同种型
IgG应用
FCM, ICC, WB反应种属 ?
Hu纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 |
|---|---|
| WB | 1:1000 |
| ICC | 1:500 |
| FCM | 1:50 |
Herpesvirus entry mediator (HVEM), also known as tumor necrosis factor receptor superfamily member 14 (TNFRSF14), is a human cell surface receptor of the TNF-receptor superfamily encoded by the TNFRSF14 gene. Mutations in this protein have been recurrently been associated to cases of diffuse large B-cell lymphoma and pediatric-type follicular lymphoma. This receptor was identified as a cellular mediator of herpes simplex virus (HSV) entry. Binding of HSV viral envelope glycoprotein D (gD) to this receptor protein has been shown to be part of the viral entry mechanism.
免疫印迹
WB result of HVEM/TNFRSF14 Rabbit mAb
Primary antibody: HVEM/TNFRSF14 Rabbit mAb at 1/1000 dilution
Lane 1: recombinant human HVEM/TNFRSF14 protein 10 ng
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 20 kDa
Observed MW: 27, 38 kDa
(This blot was developed with high sensitivity substrate)
流式分析
Flow cytometric analysis of human PBMC (human peripheral blood mononuclear cell) labelling HVEM/TNFRSF14 antibody at 1/50 dilution (1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫细胞化学
ICC shows positive staining in Ramos cells. Anti-HVEM/TNFRSF14 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
Negative control: ICC shows negative staining in 293T cells. Anti-HVEM/TNFRSF14 antibody was used at 1/500 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
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